Bazı Hidrosollerin Gıda Kaynaklı Patojen ve Bozulma Etmeni Bakteriler Üzerindeki Antimikrobiyal Etkilerinin Analizi

Portakal kabuğu, nar kabuğu, şadok kabuğu, mandalina kabuğu ve kekik bitkisinden elde edilen beş hidrosolün 3 gıda kaynaklı bakteri (Staphylococcus aureus, Salmonella Parathyphi A ve Klebsiella pneumoniae) ve 3 balık bozucu bakterileri (Vibrio vulnificus, Pseudomonas luteola ve Photobacterium damselae) üzerindeki antimikrobiyal aktivitesi, mikrodilüsyon ve disk difüzyon yöntemleriyle test edildi. S. Parathyphi A, özellikle nar, kekik ve şadok kabuğundaki (10,5 mm inhibisyon zonuyla) test edilen tüm hidrosollere karşı en duyarlı bakterilerden biriydi. S. aureus' un gelişimi üzerindeki inhibisyon bölgeleri, sadece kekik ve mandalina kabuğunun hidrosollerinde gözlendi. Portakal ve mandalina kabuğundan üretilen hidrosoller, balıklarda bozulma bakterilerinin büyümesinin inhibe edilmesinde etkili olmuştur. Hidrosollerin minimum inhibitör konsantrasyon (MIC) ve minimum bakteri öldürücü konsantrasyonları (MBC) genel olarak sırasıyla 50 ve >50 mg/mL idi. Bu sonuçlar, kekik ve nar kabuğu hidrosollerinin test bakterilerine karşı en güçlü antimikrobiyal etkilere sahip olduğunu göstermektedir ancak atık hidrosollerin etkisi bakteri suşlarına bağlı olarak değişmektedir.

Testing the Antimicrobial Effects of Some Hydrosols on Food Borne-Pathogens and Spoilage Bacteria

The antimicrobial activity of five hydrosols made from orange peel, pomegranatepeel, shaddock peel, mandarin peel, and thyme plant was tested by microdilutionand disc diffusion methods against 3 foodborne bacteria (Staphylococcus aureus,Salmonella Parathyphi A and Klebsiella pneumoniae) and 3 fish spoilage bacteria(Vibrio vulnificus, Pseudomonas luteola, and Photobacterium damselae).S. Parathyphi A was one of the most susceptible bacteria against all hydrosolstested mainly pomegranate, thyme and shaddock peel (with more than 10.5 mminhibition zone). Inhibition zones on the growth of S. aureus were only observedfrom hydrosols of thyme and mandarin peel. Hydrosols made from orange andmandarin peel were effective on inhibition of the growth of fish spoilage bacteria.Minimum inhibitory concentration (MIC) and minimum bactericidalconcentration (MBC) of hydrosols were generally 50 and >50 mg/mL,respectively. These results show that thyme and pomegranate peel hydrosols hadthe strongest antimicrobial effects against test bacteria, although the effect ofwaste hydrosols vary depending on bacterial strains.

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