Elvan BAKAR, Tülin AKTAÇ, Ayşegül KABOĞLU, Hamiyet KARAKAŞ

The short-term effects of single toxic dose of citric acid in mice

Bir besin koruyucu olan sitrik asidin LD25 (480 mg/kg.va.) dozu farelere intraperitoneal yolla uygulandı. 10 gün sonra hayvanların vücut ağırlıkları, organ ağırlıkları (karaciğer, böbrek, dalak), kreatin kinaz (CK), laktat dehidrogenaz (LDH), alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) enzimlerinin serum düzeyleri ile, karaciğer dokusu üzerinde sitrik asidin etkileri araştırıldı. Otoanalizörde tayin edilen enzim aktiviteleri U/L olarak değerlendirildi. Çalışmada vücut ağırlıklarında kontrol grubuna kıyasla anlamlı bir azalma gözlenmesine rağmen (p0.05 , böbrek: p>0.05, dalak: p>0.05) ve enzim aktivitelerinde (CK: p>0.05, LDH: p>0.05, ALT: p>0.05, AST: p>0.05) anlamlı olmayan bir artış gözlendi. Karaciğerin mikroskopik incelenmesinde doku dejenerasyonu, sitoplazmik vakuolizasyon, nükleer zar çöküntüleri, piknotik nukleuslar ve hepatositlerde nekroz gibi histopatolojik değişiklikler gözlendi.

Farelerde sitrik asidin tek toksik dozunun kısa süreli etkileri

The effects of LD25 (480 mg/kg.bw.) dose of citric acid, a food preservative, were investigated on body weight, organ weights (liver, kidney, spleen), creatin kinase (CK), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzymes in the blood serum, and the liver tissue of mice after 10 days. Citric acid (to experimental groups) and physiological saline (to control groups) were given intraperitoneally. The results of enzyme activities were evaluated using autoanalyzer as IU/L. Even though significant decreases in the body weights were noted when compared to those of the control group (p0.05, kidney: p>0.05, spleen: p>0.05) and serum enzyme levels (CK: p>0.05, LDH,: p>0.05, ALT: p>0.05, AST: p>0.05). Microscopical examination of the liver showed histopathological changes depending on the citric acid. These changes were tissue degeneration, cytoplasmic vacuolisations, nuclear membrane invaginations, picnotic nucleus and necrosis of the hepatocytes.

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