The effect of exogenous superowide generator chemicals on sodA and flpA promoters expression in Lactococcus lactis
Oksijen stresine aerobik büyüme şartlarında süperoksit anyonu veya hidrojen peroksit sebep olmaktadır. Bazı bakteriler toksik oksijen türlerini SodA enzimini kullanarak elimine edebilmektedirler. Bu çalışmada süperoksit üreten kimyasalların ve hidrojen peroksidin sodA veflpA promoter aktivitesine etkisi incelenmiştir. Bulgular flpA promotor aktivitesinin menadione eklenmesi ile flpB mutant bakterisinde yükseldiği fakat flpA mutant bakterisinde yükselmediği bulunmuştur. Ayrıca sodA aktivitesi kültürün sallanması, memadione, paraquat ve plumbagin kimyasallarının eklenmesi ile sırasıyla 4-, 7-, 50, ve 27-kat yükseltilmiştir. Katalâz enzim eklenmesi flpA mutant bakterilere büyüme safhasında flpB mutantı ve mutant olmayan bakterilerden daha fazla pozitif etki sağlamıştır. Dışarıdan hidrojen peroksit eklenmesi ne sodA promotor aktivitesini ne de SodA enzim aktivitesini arttırmıştır.
Lactococcus lactis' te dış kaynaklı süperoksit üreten kimyasalların sodA ve flpA promoter aktivitesine etkisi
Oxidative stress is caused by superoxide anion or hydrogen peroxide under aerobic growth condition. Some bacteria could eliminate toxic species of oxygen using SodA enzyme. The effect of superoxide generating chemicals and hydrogen peroxide on sodA and flpA promoter activities were investigated in this study. The observation indicated that the flpA promoter activity increased with menadione addition in the flpB mutant but not flpA mutant backgrounds. Also sodA activity increased 4-, 7-, 50, and 27-fold in shaking (250 rpm), memadione, paraquat and plumbagin addition respectfully. Catalase enzyme supplementation had positive effect on bacterial growth during exponential phase of flpA mutants than flpB mutants and wild type. Exogenously, hydrogen peroxide addition was unable to increase either sodA promoter or SodA enzyme activity.
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- ISSN: 1303-3646
- Yayın Aralığı: Yılda 2 Sayı
- Yayıncı: Haliç Üniv. Fen Edebiyat Fak.
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