Antifungal activity of some Himalayan medicinal plants using direct bioautography
Biyoâktif bileşiklerin araştırılmasında lipofilik yaprak ekstrelerinin doğrudan biyootografisi antifungal tarama için kullanıldı. Bu nedenle Himalaya halkı tarafından kullanılan tibbi bitkilerin lipofilik (diklorometan) yaprak ekstreleri araştırıldı. Biyootografide araştırma organizması olarak Alternaria alternata ve Curvularia lünata kullanıldı. Sonuçlarda mantar büyümesinde inhibisyon bölgesinin çapı değerlendirildi, araştırılan 12 bitki türünden beşinde antifungal aktivite belirlendi. Silika gel G levhaların yürütülmesinde CHC13-CH3OH (1:9, v/v) solvent olarak kullanıldı. Vitex negundo (Rf değeri 0.85), Zantoxylum alatum (Rf değeri 0.86), ipomea carnea (Rf değeri 0.86), Thuja orientalis (Rf değeri 0.80) ve Cinnamomum camphora (Rf değeri 0.89) lipofilik ekstreleri için belirgin inhibisyon bölgeleri saptandı. En iyi antifungal aktivite lipofilik T. orientalis yaprak ekstrelerinde gösterildi.
Bazı himalaya tıbbi bitkilerinin antifungal aktivitelerinin doğrudan biyootografi kullanılarak gösterilmesi
In the search for bioactive compounds, direct bioautography of plant lipophilic leaf extracts was used in antifungal screening. Thus, lipophilic (dichloromethane) leaf extract of medicinal plants used by Himalayan people were investigated. Alternaria alternata and Curvularia lunata were used as test organism in bioautography. The results, evaluated by the diameter of the inhibition zone of fungal growth, indicate that five plant species, among the 12 investigated, showed antifungal activity. CHC13 - CH3OH (1:9, v/v) was used as a solvent to develop silica gel TLC plates. Clear inhibition zones were observed for lipophilic extracts of Vitex negundo (RF value 0.85), Zantoxylum alatum (RF value 0.86), Ipomea carnea (RF value 0.86), Thuja orientalis (RF value 0.80) and Cinnamomum camphora (RF value 0.89). The best antifungal activity was shown by lipophilic leaf extract of T. orientalis.
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- ISSN: 1303-3646
- Yayın Aralığı: Yılda 2 Sayı
- Yayıncı: Haliç Üniv. Fen Edebiyat Fak.
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