Hassan GHARDINEJAD, Jianbo CHANG, Bin ZHU

Study on the population genetic of the great sturgeon (Huso huso) in the southern part of the Caspian Sea using microsatellite loci

Fourteen sets of microsatellites primers were tested on randomly selected individuals of great sturgeon, H. huso. Five sets were developed for lake sturgeon (Afu-19, 34, 39, 54, and 68) and seven sets were developed for Adriatic sturgeon (An-0, 1, 16, 20, 40, 76, and 77) and fi nally two sets were developed for Chinese sturgeon (As-73, and 74). Except two sets of Adriatic sturgeon primers, An-76, and An-16, the rest of primers reproducibly amplifi ed the beluga sturgeon DNA. Five pairs of primers out of 14 have been selected and confi rmed to be used for separating the populations. Those primers are; Afu-19, Afu-39, Afu-54 from lake sturgeon; As-73 from Chinese sturgeon, and An-77 from Adriatic sturgeon. The samples from the east region of the southern Caspian Sea did not amplify at Afu-19, Afu-39, Afu-54, and An-77 loci. However, samples from middle and west regions of the Caspian Sea did amplify at Afu-19, Afu-39, Afu-54, An-77 loci in different manners of producing the bands. Samples from all three regions did amplify at As-77 locus. However, the bands at the samples from the east region are distributed differently from the bands at the samples from the middle and west regions. Statistical analysis revealed that the number of alleles per locus ranged from 6 to 11, and one locus had at least six alleles. Population differentiation combination test (Fisher's method) based on both genic differentiation and on genotypic differentiation (G-based) for the two sets of samples demonstrated that the probabilities are highly signifi cant. It means possibly two great sturgeon populations exist in the southern part of the Caspian Sea.

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[1] Birstein, V. J.; Vasiliev, V. P., 1987: Tetraploid-octaploid relationships and karyological evolution in the order Acipenserformes (Pisces). Karyotypes, nucleoli, and nucleolus-organizer region in four acipenserid species. Genetica 73: 3-12.
[2] Birstein, V. J.; Hanner, R.; DeSalle, R., 1997: Phylogeny of the Acipenseriformes: cytogenetic and molecular approaches, pp. 127-155 in sturgeon biodiversity and conservation, edited by V. J. Birstein, J. R. Waldman and W. E. Bemis. Kluwer Academic Publishing, Dordrecht, the Netherlands.
[3] Christians.1991. Conservation of polymorphic simple sequence loci in cetacean species. Nature, 354: 63-65.
[4] Davis, C. S.; Strobeck, C., 1998: IsolationVariability, and cross species amplifi cation of polymorphic microsatellite loci in the family Mustelidae. Mol. Ecol. 7, 1776-1778.
[5] Dingerkus, G.; Howell, W. M., 1976: Karyotypic analysis and evidence of tetraploidy in the North American paddlefi sh, polyodon spathula. Science. 194: 842-844.
[6] Fontana, F., 1994: Chromosomal nucleolar organizer regions in four sturgeon species as markers of karyotype evolution in Acipenseriformes (Pisces). Genome 37: 888-892.
[7] Fontana, F.; Lanfredi, M.; Chicca, M.; Aiello, V. and Rossi, R., 1998a: Localization of the repetitive telomeric sequence (TTAGGG)n in four sturgeon species. Chromosome Res. 6: 303-306.
[8] Fontana, F.; Tagliavini, J.; Gongiu, L.; Lanfredi, M.; Chicca, M. et al., 1998b: Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fl uorescent in situ hybridization. Mar. Biol. 132: 495-501.
[9] Jenneckens, I. 1999; Investigation on the suitability of biochemical and molecular markers for an identifi cation of different sturgeon species as well as their hybrids. Ph.D. Thesis, University of Gottingen, Gottingen, Germany.
[10] Ludwig, A.; Belfi ore, N. M.; Pitra, C.; Svirsky, V.; Jenneckens, I., 2001: Genome duplication events and functional reduction of ploidy levels in sturgeon (Acipenser, Huso, and Scaphirhynchus). Gen. Soci. Amer. 158: 1203-1215).
[11] May, B.; Krueger, C. C.; Kincaid, H. L., 1997: Genetic variation at microsatellite loci in sturgeon: primer sequence homology in Acipenser and Scaphirhynchus. Can. J. Fish. Aquat. Sci. 54, 1542-1547.
[12] McQuown, E.; Gall, G. A. E.; May, B., 2002: Characterization and inheritance of six microsatellite loci in lake sturgeon (acipenser fulvescens). Trans. Am. Fish. Soc. 131, 299-307.
[13] Ohno, S.; Muramoto, J.; Stenius, C.; Christian, L.; Kittrell, W. A. et al., 1969. Microchromosomes in holocephalian, chondrostean and holostean fi shes. Chromosoma 26: 35-40.
[14] Pyatskowit, J.; Krueger, C. C.; Kincaic, H. L.; May, B., 2001: Inheritance on microsatellite loci in the polyploidy derivative lake sturgeon (Acipenser fulvescens). Genome 44, 185-191.
[15] Samadi, S.; Mavarez, J.; Pointier, J.; Delay, B.; Jarne, P., 1999: Microsatellite and morphological analysis of population structure in the parthenogenetic freshwater snail Melanoides tuberculata: insights into the creation of clonal variability. Mol. Ecol. 8, 1141-1153.
[16] Smith, C. T.; Koop, B. F.; Nelson, R. J., 1998: Isolation and characterization of coho salmon (Oncorhynchus kisutch) microsatellites and their use in other salmonids. Mol. Ecol. 7, 1614-1615.
[17] Tagliavini, J.; Williot, P.; Congius, L. Chicca, M.; Lanferedi M. et al., 1999. Molecular cytogenetic analysis of the karyotype of the European Atlantic sturgeon, Acipenser sturio. Heredity 83: 520-525.
[18] Zane, L.; Patarnello, T; Ludwig, A.; Fontana, F.; Congiu, L., 2002: Isolation and characterization of microsattelites in the Adriatic sturgeon (Acipenser naccarii). Mol. Ecol. Not. (2002) 2, 586-588.
[19] Zhu, B.; Zhou, F.; Cao, H.; Shao, Z.; May, B. Chang, J., 2002: Analysis of genetic variation in the Chinese sturgeon, Acipenser sinensis: estimating the contribution of artifi cially produced larvae in a wild population. Appl. Ichthyol. 18 (2002), 301-306.
[20] Zhu, B.; Liao, X.; Shao, Z.; Rosenthal, H.; Chang, J., 2005. Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis: Mol. Ecol. Not. 2005.