Effect of carbon source on production of lytic enzymes by Trichoderma harzianum

We investigated the effect of the different carbon sources and phytopathogenic fungal cell walls on production of enzymes by Turkish strain Trichoderma harzianum T15. Trichoderma harzianum T15 secretes β-1,3-glucanase and chitinase in the presence of different carbon sources. Maximal β-1,3-glucanases activity secreted was detected in media supplemented laminarin or Dreschlera sorokiniana and Sclerotium rolfsii purifi ed cell walls. The highest chitinase activity was obtained in medium supplemented with chitin or D. sorokiniana and S. rolfsii purifi ed cell walls. The properties of this enzymes suggest that the enzymes might play different roles in host cell wall lysis during mycoparasitism.

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