In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

Leaf explants of a semi dwarf cultivar of pomegranate were placed on Murashige and Skoog [6] (MS) medium supplemented with various concentrations of 6-benzyl adenin (BA) and naphthalene acetic acid (NAA) for callus induction. Maximum callus induction was observed on a media containing 1 mg L-1 BA with 0 mg L-1 NAA. However, the highest callus growth was obtained on a medium containing 1 mg L-1 BA and 0.5 mg L-1 NAA.. Maximum shoot proliferation was observed when shoots were cultured on woody plant medium (WPM) supplemented with 2 mg L-1 BA. In this treatment, after 4 subcultures, 85 shoots were produced from one original explant. Among treatments used in rooting experiments, shoots cultured on WPM medium with 0-0.2 mg L-1 indol butyric acid (IBA) had the maximum root percentage (100%). Rooted plantlets were cultured in a soil mixture containing vermiculite (60%), perlite (30%) and coco peat (10%) v/v. After 2 months, 100% of plants survived and transferred to the greenhouse
Journal of Applied Biological Sciences-Cover
  • ISSN: 1307-1130
  • Başlangıç: 2007
  • Yayıncı: Nobel Bilim ve Araştırma Merkezi
Sayıdaki Diğer Makaleler

Determining the Priority of Effective Factors on Forest Fire from Analytical Hierarchy Process

Mehran NASIRI

Evaulation of Water Quality By Using Trophic Diatom Index: Exaple of Porsuk Dam Lake

Cem TOKATLI

Evaluation of Qualitive and Quantitive Traits of Canola (Brassica Napus L.) in Different Sowing Dates and Regions

Reza AMIRNIA, Mahdi BAYAT, Asadollah GHOLAMIAN

Antibiotic Levels and heavy metal resistance in gram-negative bacteria isolated from seawater, Iskenderun Organized Industrial Zone

Tamer AKKAN, Ayşenur KAYA, Sadık DİNÇER

High Salt Induced Oxidative Damage and Antioxidant Response Differs in Nicotiana tabacum L. and Nicotiana rustica L. Cultivars

Özlem Darcansoy İŞERI, Didem Aksoy KÖRPE, Feride İffet SAHIN, Mehmet HABERAL

In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

Alireza BONYANPOUR, Morteza KHOSH-KHUI

Expression Pattern of Human ADAMTS-2 (A disintegrin and metalloproteinase with thrombospondin motif, 2) in Different Cell Lines

Meltem ALPER, Feray KÖÇKAR

Chemical and Bacteriological Properties of Fresh Water Fountains of Karaman Province

Numan Emre GÜMÜŞ, Mehmet KARATAŞ, Cengiz AKKÖZ

Effect of antitranspirants application on yield and yield components in soybean (Glycine max L.) under limited irrigation

Mina JAVAN, Mehdi TAJBAKHSH, Babak Abdollahi MANDOULAKANI

Enzyme activities of the marine-derived fungus Alternaria alternata cultivated on selected agricultural wastes

A. Mostafa FATEN, A. Abd El Aty ABEER