Alireza BONYANPOUR, Morteza KHOSH-KHUI

127161

In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

Leaf explants of a semi dwarf cultivar of pomegranate were placed on Murashige and Skoog [6] (MS) medium supplemented with various concentrations of 6-benzyl adenin (BA) and naphthalene acetic acid (NAA) for callus induction. Maximum callus induction was observed on a media containing 1 mg L-1 BA with 0 mg L-1 NAA. However, the highest callus growth was obtained on a medium containing 1 mg L-1 BA and 0.5 mg L-1 NAA.. Maximum shoot proliferation was observed when shoots were cultured on woody plant medium (WPM) supplemented with 2 mg L-1 BA. In this treatment, after 4 subcultures, 85 shoots were produced from one original explant. Among treatments used in rooting experiments, shoots cultured on WPM medium with 0-0.2 mg L-1 indol butyric acid (IBA) had the maximum root percentage (100%). Rooted plantlets were cultured in a soil mixture containing vermiculite (60%), perlite (30%) and coco peat (10%) v/v. After 2 months, 100% of plants survived and transferred to the greenhouse
Keywords:

Callus induction, pomegranate, Rooting Shoots proliferation, Tissue culture,

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Journal of Applied Biological Sciences-Cover
  • ISSN: 1307-1130
  • Yayın Aralığı: 6
  • Başlangıç: 2007
  • Yayıncı: Nobel Bilim ve Araştırma Merkezi
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