Gram-negative bacteria were isolated from the territorial seawater of Iskenderun Organized Industrial Zone in Iskenderun Bay, to measure their resistance levels against to an tibiotic an d heavy metal. Sixteen species o f b acteria were identified b y using VITEK II System. Total of 18 antibiotics disc and four heavy metals were applied for the resistance test. Antibiotic resistances of all isolates were at high percentages for eritromisine (94.4%), ampicilline (72.7%), streptomycin (68.3%), cefazolin (64.6%) and carboksipeneme (57.1%). The highest resistant strain was resistant against to 17 antibiotics, while the weakest resistant of two isolates were sensitive to all antibiotics. MAR index values were found to be higher than 0.2 for 94.9% of all isolates. Heavy metal resistances of all isolates were found to be in high percentages as 100% for cadmium, %100 for copper, 90.7% for manganese, and 67.7 % for lead. High r esistances o f e xamined ba cteria a gainst t o bot h a ntibiotics a nd he avy metals i ndicated a dense a nd m ultisource pollution in the bay. The pollution in territorial waters of the bay may threat the aquatic life consequently, the public health