Gökkuşağı Alabalığında Karşılaşılan Bazı Bakteriyel Hastalık Etkenlerinin Hızlı Teşhisi

Bu çalışmada, PCR yöntemi ile gökkuşağı alabalığında (Oncorhynchus mykiss) hastalığa neden olan beş farklı patojenin (Aeromonas salmonicida, Aeromonas hydophila, Yersinia ruckeri, Flavobacterium psychrophila ve Renibacterium salmoninarum) hızlı teşhis edilebilmesi için beşyüz elli balık doku (solungaç, böbrek, ağız, deri ve yüzgeç) örneğinden direk olarak saflaştırılan DNA’lar kullanılmıştır. Çalışmamızdaki balık hastalık etkenlerini tespit edebilmek için beş farklı hedef gen bölgesi (gryA, gyrB, ompTS, n-DNA ve YER) kullanılmıştır ve bunların PCR reaksiyonları gerçekleştirilmiştir. Çalışmamızda sonuç olarak, hastalıklara neden olan etken bakteriler, dokulardan izole edilen DNA’lardaki hedef genlerin PCR methodu kullanılarak tespit edilmesiyle 3-4 saat içerisinde tanımlanmıştır.

Anahtar Kelimeler:

PCR, Balık hastalığı

Rapid Detection of Some Bacterial Diseases in Rainbow Trout

In this study, we used five hundred fifty different tissue samples (gill, kidney, mouth, skin and finn) to detect five pathogens (A. salmonicida, A. hydophila, Y. ruckeri, F. psychrophila and R. salmoninarum) in rainbow trout (Oncorhynchus mykiss) with using direct PCR methods. Five targeted genes (gryA, gyrB, ompTS, n-DNA and YER) have been chosen to detect the causative agents of diseases. As a result, five infection agents of rainbow trout were identified and determinated within 3-4 hours time by using direct PCR from fish tissues, without any microbiological examination methods.

Keywords:

PCR, Fish Diseases,

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