Çeşitli zayıf organik asitler ve kombinasyonlarının saccharomyces cerevisiae'ye karşı antifungal etkileri

Zayıf organik asitlerin S. cerevisiae hücrelerine karşı antifungal etkinliği incelenmiştir. Zayıf organik asitler olarak, hekzanoik (C6), oktanoik (C8), dekanoik (C10) ve benzoik asitlerin Minimum İnhibitör Konsantrasyon (MİK) ve inhibisyon bölgesi ölçümleri ile belirlenmiştir. MİK sonuçlarına göre maya hücrelerine karşı en etkili asit, dekanoik asittir (MİK: 0,2-0,3 mM). Bahsi geçen zayıf asitlerin inhibisyon mekanizmalarını anlayabilmek için, ekstraselüler ortam pH ölçümleri yapılmıştır. Ekstraselüler pH’daki düşüş; pH’da aynı miktarda düşüşe neden olan hidroklorik asit (HCl) ile karşılaştırılmıştır. Sonuçlar, maya hücrelerine karşı zayıf asitlerin etkinliklerinin sadece asitlikten kaynaklı olmadığını, ancak anyonların toksik etkisi ve zayıf asitlerin hücresel membran içine sızmasının rol oynayabileceğini göstermiştir. Buna ek olarak, zayıf asitlerin sinerjik etkileri incelenmiş ve bu zayıf asitlerin kombinasyolarının tek başına kullanımlara göre daha etkili olduğu gözlemlenmiştir. Çalışmada, zayıf asitlerin antifungal aktivite mekanizmalarına genel olarak bir açıklama getirmekle birlikte farklı konsantrasyonlarda kombinasyon halinde kullanımları da incelenmiştir.

Anahtar Kelimeler:

Saccharomyces cerevisiae, Zayıf organik asitler, Antifungal aktivite, Kombinasyon

Antifungal activity of various weak organic acids and their combinations against saccharomyces cerevisiae

Antifungal activity of four weak organic acids against S. cerevisiae cells was examined. Antifungal effects of hexanoic (C6), octanoic (C8), decanoic (C10) and benzoic acids were determined through Minimum Inhibitory Concentration (MIC), and inhibition zone measurements. The most effective weak acid was decanoic acid (MIC: 0.2-0.3 mM) according to MIC results. In order to have some insight into the inhibition mechanism of these weak acids, their efficiency was compared with that of hydrochloric acid (HCl) giving the same amount of drop in extracellular pH. Results demonstrated that the inhibition of yeast cells by weak acids is not simply due to acidity, but toxic effect of the anion and the insertion of the weak acids inside the cellular membrane may play a role. Moreover, synergistic effects of weak acids were examined, and it has been shown that combinations of weak acids are more effective than using weak acids alone. Thus, this research not only opens new perspectives on antifungal activity mechanisms of weak acids but also help their usage in combination widely.

Keywords:

Saccharomyces cerevisiae, Weak organic acids, Antifungal activity, Combination,

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