Şükrü DEĞİRMENÇAY, M. Sinan AKTAŞ, Başak HANEDAN, Akın KIRBAŞ, Nergis ULAŞ, Emre YANAR, Ömer AYDIN, Emre EREN, Muhammed Sertaç EROĞLU

A study of lipid and protein profiles and liver enzyme levels in neonatal diarrhea calves based on clinical severity of the disease

Anahtar Kelimeler:

Calf diarrhea, liver enzymes, lipid profile, protein profile

A study of lipid and protein profiles and liver enzyme levels in neonatal diarrhea calves based on clinical severity of the disease

The purpose of this study was to investigate the serum lipid and protein profile as well as liver enzyme levels in neonatal calves with diarrhea. The study included 40 calves, 30 with diarrhea and 10 healthy (control). Calves with diarrhea were divided into three groups based on clinical findings: mild, moderate, and severe. Blood samples were taken from the vena jugularis after routine clinical examinations of the calves to determine the lipid profile, protein profile, liver enzyme levels, and glucose levels. Serum total cholesterol (TC) (P<0.01), HDL (P<0.01), and LDL (P<0.05) levels were all found to be lower in the calves in the mild, moderate, and severe groups compared to the control group. No significant difference in TP and ALB values was found between the groups. Additionally, serum aspartate aminotransferase (AST) (P<0.01), alkaline phosphatase (ALP), and glucose (P<0.05) levels were also greater in calves with diarrhea compared to the control group. The current study concluded that there was no change in the protein profile in calves with neonatal diarrhea, but the lipid profile was negatively affected and liver function was impaired, and the impairment in liver function increased as the clinical severity of the disease increased.

Keywords:

Calf diarrhea, liver enzymes, lipid profile, protein profile,

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Akgün, S., Ertel, N. H., Mosenthal, A., & Oser, W. (1998). Postsurgical reduction of serum lipoproteins: Interleukin-6 and the acute-phase response. Journal of Laboratory and Clinical Medicine, 131: 103-108. DOI: 10.1016/s0022-2143(98)90083-x
Al-Alo, K. Z. K., Nikbakht Brujeni, G., Lotfollahzadeh, S., Moosakhani, F., & Gharabaghi, A. (2018). Correlation between neonatal calf diarrhea and the level of maternally derived antibodies. Iranian Journal of Veterinary Research, 19(1), 3-8
Albayrak, H., & Kabu, M. (2016). Determining serum haptoglobin and cytokine concentrations in diarrheic calves. Fırat University Veterinary Journal of Health Sciences, 30 (2), 113-117.
Alvarez, C., & Ramos, A. (1986). Lipids, lipoproteins, and apoproteins in serum during infection. Clinical Chemistry, 32 (1 Pt 1): 142-145.
Anderson, N. L., & Anderson, N. G. (2002). The human plasma proteome. Molecular and Cellular Proteomics, 1(11), 845-867. DOI: 10.1074/mcp.r200007-mcp200
Arfuso, F., Fazio, F., Panzera, M., Giannetto, C., di Pietro, S., Giudice, E., & Piccione, G. (2017). Lipid and lipoprotein profile changes in newborn calves in response to the perinatal period. Acta Veterinaria Beograd, 67 (1), 25-32. https://doi.org/10.1515/acve-2017-0003
Athanasiou, L. V., Katsoulos, P. D., Ziogas, C., Kassab, A., & Polizopoulou, Z. (2019). Serum protein electrophoretic profile in diarrheic neonatal calves. Comparative Clinical Pathology, 28 (3), 685-688. https://doi.org/10.1007/s00580-019-02935-w
Aydogdu, U., Coskun, A., Yildiz, R., Guzelbektes, H., & Sen, I. (2018). Clinical importance of lipid profile in neonatal calves with sepsis. The Journal of the Hellenic Veterinary Medical Society, 69 (4), 1189-1194. https://doi.org/10.12681/jhvms.15926
Ballou, M. A., Cobb, C. J., Hulbert, L. E., & Carroll, J. A. (2011). Effects of intravenous Escherichia coli dose on the pathophysiological response of colostrum-fed Jersey calves. Veterinary Immunology and Immunopathology, 141 (1–2), 76–83. DOI: 10.1016/j.vetimm.2011.02.008.
Barati, M., Nazari, M. Z., Taher, M.T., & Farhadi, N. (2011). Comparison of lipid profile in septic and non-septic patients. Iranian Journal of Clinical Infectious Diseases, 6 (4), 144-147.
Bartosz, J., & Katarzyna, A. (2016). Application of native agarose gel electrophoresis of serum proteins in veterinary diagnostics. Journal of Veterinary Research, 60: 501–508. DOI: 10.1515/jvetres-2016-0074
Başer, D. F., & Civelek, T. (2013). Correlations between venous acid-base status and renal function in neonatal calves with acute diarrhea. Kocatepe Veterinary Journal, 6 (1), 25-31. DOI: 10.5578/kvj.5516 Bozukluhan, K., Merhan, O., Gokce, H. I., Deveci, H. A., Gokce, G., Ogun, M., & Marasli, S. (2017). Alterations in lipid profile in neonatal calves affected by diarrhea. Veterinary World, 10 (7), 786-789. DOI: 10.14202/vetworld.2017.786-789
Cho, Y. I., & Yoon, K. J. (2014). An overview of calf diarrhea - infectious etiology, diagnosis, and intervention. Journal of Veterinary Science, 15 (1), 1-17. DOI: 10.4142/jvs.2014.15.1.1
Cirstea, M., Walley, K. R., Russell, J. A., Brunham, L. R., Genga, K. R., & Boyd, J. H. (2017). Decreased high-density lipoprotein cholesterol level is an early prognostic marker for organ dysfunction and death in patients with suspected sepsis. Journal of Critical Care, 38: 289-294. DOI: 10.1016/j.jcrc.2016.11.041
Civelek, T., Kav, K., Camkerten, I., Celik, H. A., & Acar, A. (2007). Effects of bacterial pneumonia in neonatal calves on serum lipids. Bulletin of the Veterinary Institute in Puławy, 51 (4), 503-507.
Constable, P. D., Hinchcliff, K. W., Done, S. H., & Grünberg, W. (2017). Neonatal infection disease. Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs, and Goats. 11th ed. Elsevier, China. p1874-1903.
El-Bahr, S. M., & EL-Deeb, W. M. (2013). Acute phase proteins, lipid profile and proinflammatory cytokines in healthy and bronchopneumonic water buffalo calves. American Journal of Biochemistry and Biotechnology, 9 (1), 34-40. https://doi.org/10.3844/ajbbsp.2013.34.40
Fraunberger, P., Schaefer, S., Werdan, K., Walli, A. K., & Seidel, D. (1999). Reduction of circulating cholesterol and apolipoprotein levels during sepsis. Clinical Chemistry and Laboratory Medicine, 37 (3), 357-362. DOI: 10.1515/CCLM.1999.059
Gordon, B. R., Parker, T. S., Levine, D. M., Saal, S. D., Wang, J. C., Sloan, B. J., Barie, P. S., & Rubin, A. L. (2001). Relationship of hypolipidemia to cytokine concentrations and outcomes in critically ill surgical patients. Critical Care Medicine, 29 (8), 1563–1568. https://doi.org/10.1097/00003246-200108000-00011
Grodzki, K., Lechowski, R., & Lenarcik, M. (1991). The biochemical profile of calves' liver in the course of diarrhea during the first 10 days of life. Polskie Archiwum Weterynaryjne, 31 (3-4), 49-63.
Grünberg, W. (2022, October 12). Diarrhea in neonatal ruminants. https://www.msdvetmanual.com/digestive-system/intestinal-diseases-in-ruminants/diarrhea-in-neonatal-ruminants
Hardaróttir, I., Grünfeld, C., & Feingold, K. R. (1994). Effects of endotoxin and cytokines on lipid metabolism. Current Opinion in Lipidology, 5 (3), 207–215. https://doi.org/10.1097/00041433-199405030-00008
Humblet, M. F., Coghe, J., Lekeux, P., & Godeau, J. M. (2004). Acute phase proteins assessment for an early selection of treatments in growing calves suffering frombronchopneumonia under field conditions. Research in Veterinary Science, 77 (1), 41–47. https://doi.org/10.1016/j.rvsc.2004.02.009
Irmak, K., & Güzelbekteş, H. (2003). Alteration in some hematological and biochemical parameters in the calves with presumed septic shock. Kafkas Universitesi Veteriner Fakültesi Dergisi, 9 (1): 53-57.
Izzo, M. M., Kirkland, P. D., Mohler, V. L., Perkins, N. R., Gunn, A. A., & House, J. K. (2011). Prevalence of major enteric pathogens in Australian dairy calves with diarrhoea. Australian Veterinary Journal, 89 (5), 167–173. https://doi.org/10.1111/j.1751-0813.2011.00692.x
Jacobsen, S., Andersen, P. H., Toelboell, T., & Heegaard, P. M. (2004). Dose dependency and individual variability of the lipopolysaccharide-induced bovine acute phase protein response. Journal of Dairy Science, 87(10), 3330–3339. https://doi.org/10.3168/jds.S0022-0302(04)73469-4
Joshi, V., Gupta, V. K., Dimri, U., Mandal, R. S. K., & Sharma, D. K. (2015). Serum lipid profile in bacterial Bovine Respiratory Disease (BRD) affected calves. Intas Polivet, 16 (2), 187-188.
Kaneko, J. J., Harvey, J. W. & Bruss, M.L. (2008). Clinical Biochemistry of Domestic Animals. 6th ed. Academic Press, New York. (pp. 364-390).
Khovidhunkit, W., Memon, R. A., Feingold, K. R., & Grunfeld, C. (2000). Infection and inflammation-induced proatherogenic changes of lipoproteins. The Journal of Infectious Diseases, 181(Supplement_3), S462-S472. DOI: 10.1086/315611
Knowles, T. G., Brown, S. N., Edwards, J. E., Phillips, A. J., & Warriss, P. D. (1999). Effect on young calves of a one‐hour feeding stop during a 19‐hour road journey. Veterinary Record, 144 (25), 687-692. https://doi.org/10.1136/vr.144.25.687
Lekkou, A., Mouzaki, A., Siagris, D., Ravani, I., & Gogos, C. A. (2014). Serum lipid profile, cytokine production, and clinical outcome in patients with severe sepsis. Journal of Critical Care, 29 (5), 723-727. https://doi.org/10.1016/j.jcrc.2014.04.018
Marcato, F., Van den Brand, H., Kemp, B., & Van Reenen, K. (2018). Evaluating potential biomarkers of health and performance in veal calves. Frontiers in Veterinary Science, 5, 133. https://doi.org/10.3389/fvets.2018.00133
Merhan, O., Bozukluhan, K., Gökçe, G., & Yılmaz, O. (2016). Investigation on the levels of haptoglobin, ceruloplasmin and some biochemical parameters levels in calves with diarrhea. Sağlık Bilimleri Veteriner Dergisi, Fırat Üniversitesi, 30 (3), 195-198. DOI: 10.14202/vetworld.2018.1055-1058
Morin, E. E., Guo, L., Schwendeman, A., & Li, X. A. (2015). HDL in sepsis–risk factor and therapeutic approach. Frontiers in Pharmacology, 6, 244. https://doi.org/10.3389/fphar.2015.00244
Murch, O., Collin, M., Hinds, C. J., & Thiemermann, C. (2007). Lipoproteins in inflammation and sepsis. I. Basic science. Intensive Care Medicine, 33 (1), 13-24. DOI: 10.1007/s00134-006-0432-y
Nagyová, V., Šoltésová, H., Tóthová, C., & Nagy, O. (2015). The impact of respiratory disease and diarrhoea on protein profiles in calves. Folia Veterinaria, 59 (1), 5-11.
Nassaji, M., & Ghorbani, R. (2012). Plasma lipid levels in patients with acute bacterial infections. Turkish Journal of Medical Sciences, 42 (3), 465-469. https://doi.org/10.3906/sag-1011-1278
Pieper, R., Gatlin, C. L., Makusky, A. J., Russo, P. S., Schatz, C. R., Miller, S. S., ... & Steiner, S. (2003). The human serum proteome: Display of nearly 3700 chromatographically separated protein spots on two‐dimensional electrophoresis gels and identification of 325 distinct proteins. PROTEOMICS: International Edition, 3 (7), 1345-1364. DOI: 10.1002/pmic.200300449
Ramachandran, G. (2014). Gram-positive and gram-negative bacterial toxins in sepsis: a brief review. Virulence, 5 (1), 213-218. DOI: 10.4161/viru.27024
Russell, K. E., & Roussel, A. J. (2007). Evaluation of the ruminant serum chemistry profile. Veterinary Clinics of North America: Food Animal Practice, 23 (3), 403-426. https://doi.org/10.1016/j.cvfa.2007.07.003
Sayers, R. G., Kennedy, A., Krump, L., Sayers, G. P., & Kennedy, E. (2016). An observational study using blood gas analysis to assess neonatal calf diarrhea and subsequent recovery with a European Commission-compliant oral electrolyte solution. Journal of Dairy Science, 99 (6), 4647-4655. DOI: 10.3168/ jds.2015-10600
Schneider, A., Corrêa, M. N., & Butler, W. R. (2013). Acute phase proteins in Holstein cows diagnosed with uterine infection. Research in Veterinary Science, 95 (1), 269-271. https://doi.org/10.1016/j.rvsc.2013.02.010
Sobiech, P., Rekawek, W., Ali, M., Targonski, R., Zarczynska, K., Snarska, A., & Stopyra, A. (2013). Changes in blood acid-base balance parameters and coagulation profile during diarrhea in calves. Polish Journal of Veterinary Sciences, 16 (3). DOI 10.2478/pjvs-2013-0075
Swanson, J., & Morrow-Tesch, J. (2001). Cattle transport: historical, research, and future perspectives. Journal of Animal Science, 79 (suppl_E), E102-E109. DOI:10.2527/JAS2001.79E-SUPPLE102X
Tall, A. R., & Yvan-Charvet, L. (2015). Cholesterol, inflammation and innate immunity. Nature Reviews Immunology, 15 (2), 104-116. DOI: 10.1038/nri3793
Tóthová, C., Nagy, O., Kováč, G., & Nagyová, V. (2016). Changes in the concentrations of serum proteins in calves during the first month of life. Journal of Applied Animal Research, 44 (1), 338-346. https://doi.org/10.1080/09712119.2015.1031791
Walker, P. G., Constable, P. D., Morin, D. E., Drackley, J. K., Foreman, J. H., & Thurmon, J. C. (1998). A reliable, practical, and economical protocol for inducing diarrhea and severe dehydration in the neonatal calf. Canadian Journal of Veterinary Research, 62 (3), 205.
Wendel, M., Paul, R., & Heller, A. R. (2007). Lipoproteins in inflammation and sepsis. II. Clinical aspects. Intensive Care Medicine, 33 (1), 25-35. DOI: 10.1007/s00134-006-0433-x
Yilmaz, Z., & Senturk, S. (2007). Characterization of lipid profiles in dogs with parvoviral enteritis. Journal of Small Animal Practice, 48 (11), 643-650. DOI: 10.1111/j.1748-5827.2007.00391.x