Tavuk etleri ve tavuklardan izole edilen Salmolella entrica subsp. entrica serovar Enteritidis suşlarının faj tiplendirilmesi ve pulsed-field gel electrophoresis ile analizi

Bu çalışma tavuk ve tavuk etlerinden izole edilen Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) suşlarının Pulsed-Field Gel Electrophoresis (PFGE) ve Faj tiplendirme yöntemleriyle tiplendirilmesi amacıyla yapıldı. Toplam 38 S. Enteritidis suşu XbaI restriksiyon enzimi kullanılarak PFGE yöntemiyle tiplendirildi. Tiplendirme sonucunda 7 farklı bant profili elde edildi. Analiz edilen 38 S. Enteritidis suşunun 27 sinde (%71) aynı PFGE bant profili (XI) saptandı. Tüm suşlar arasındaki genetik benzerlik % 85 bulundu. Faj tiplendirme sonucunda 38 suşun 15’i (% 39.4) S. Enteritidis PT4 (Faj Tip 4) olarak tiplendirildi. Suşların % 18.4’i PT7, % 13.1’i PT16, % 10.5’i PT1, % 7.8’i PT6 ve % 2.6’sı PT35 faj tipinde bulundu. Suşların 3’ünün faj tipi belirlenemedi. Farklı faj tipindeki (PT1, 4, 6 ve 7) suşlarda aynı PFGE profili (X1) saptandı. Bu çalışmanın sonuçları Türkiye’de tavuk orijinli S. Enteritidis suşları arasında bir genetik ilişkinin olduğunu göstermektedir. İnsan ve hayvan izolatları arasındaki genetik ilişkiyi ortaya koymak için ilave çalışmalara ihtiyaç duyulmaktadır.

Analysis of Salmonella enterica subsp, enterica serovar Enteritidis strains isolated from chickens and chicken meat by pulsed-field gel electrophoresis and phage typing

The aim of this study was to analyse S. Enteritidis strains isolated from chickens and chicken meat by Pulsed-Field Gel Electrophoresis (PFGE) and phage typing. A total of 38 S. Enteritidis strains were analysed by PFGE after digestion of DNA with XbaI restriction enzyme. Seven different PFGE profiles were observed. A predominant type (X1) was found in 27 (71 %) of 38 S. Enteritidis strains. For all the strains, the genetic similarity was 85 %. The most common phage type was PT4, which was found in 15 (39.4 %) of the strains. Other phage types were PT7 (18.4 %), PT16 (13.1 %), PT1(10.5 %), PT6 (7.8 %) and PT35 (2.6 %). Three strains were RDNC (reacted but did not conform). Different phage types such as PT1, 4, 6 and 7 belonged to the same PFGE pattern (X1). The results of this study shows that S. Enteritidis strains originated from chickens in Turkey are genetically related. Further studies should be performed to determine genetic relationships among animal and human isolates.

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