Ufuk KAMBER, Göknur TERZİ, Sena ÇENESİZ, Cevat NİSBET

Detection of vitamin $B_ {12}$ in different cow milk using ELISA technique

$B_ {12}$ vitamini, B kompleks vitaminlerin bir üyesidir. Bu vitamin kobalt içerdiğinden dolayı kobalamin olarak adlandırılmaktadır. B12 vitamini balık, et, tavuk, yumurta ve süt ürünlerinde bulunur ve bakteriler tarafından sentezlenir. $B_ {12}$ vitamini yetersizliğine bağlı olarak mide ve bağırsak bozukluklar, kol ve bacaklarda uyuşukluk ve megolablastik anemi şekillenir. Bu çalışmada farklı sığır ırklarına ait süt örneklerinde B12 vitamin düzeyleri araştırıldı. Bu amaçla Samsun ve Kars bölgesinden Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örnekleri toplandı. Süt örneklerinde $B_ {12}$ vitamini miktarı ELISA yöntemi ile tespit edildi.$B_ {12}$ vitamini Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örneklerinde sırasıyla 0.98µg/L, 1.15µg/L, 2.93µg/L ve 1.56µg/L olarak bulundu. Yapılan istatistik çalışması sonucunda vitamin $B_ {12}$miktarı Zavot ırkına ait süt örneklerinde diğer ırklardan daha yüksek bulundu (p

Farklı sığır ırklarına ait süt örneklerinde $B_ {12}$ vitamin düzeyinin ELISA tekniği ile tespiti

Vitamin B12 is a member of the vitamin B complex. It contains cobalt, and is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in fish, meat, poultry, eggs, milk and milk products. Vitamin B_ {12} deficiency have an underlying stomach or intestinal disorder that limits the absorption of vitamin B12 and might be caused to megaloblastic anemia. In this study cow milk samples were collected from in two different region in Turkey. In milk samples the amount of vitamin B_ {12} was detected by using ELISA. The vitamin B_ {12} concentrations of milk samples obtained from the cows of Holstein, Jersey, Zavot and East Anatolian Red species were determined as 0.98µg/L, 1.15µg/L, 2.93µg/L and 1.56µg/L, respectively. The concentrations of vitamin B12 in Zavot cows milk were higher than the other species.

PDF

___

1. Alais, C.: Science du lait. Principes des techniques laitières (Ed. Sepaic) Paris, 1984.
2. Alpan, O.: The breeding and production of cattle. Ankara University Veterinary Faculty Zootechny Dept, Ankara, 1992.
3. Anonymous: http://www.tarim.gov.tr, 2007.
4. Bernard, M.A., Nakonezny, P.A., Kashner, T.M.: The effect of vitamin B12 deficiency on older veterans and its relationship to health. J. Am Geriatr Soc, 1998; 46: 1199- 1206.
5. Bestuzheva, K.T.: Chemical composition of camel milk and colostrum. Konevodstvoi Konnyi Sport, 1964; 34: 19.
6. Bottiglieri, T.: Folate, vitamin B12, and neuropsychiatric disorders. Nutr Rev, 1996; 54: 382- 390.
7. Combs, G.: Vitamin B12 in The Vitamins. Academic Press, New York, 1992.
8. Dagnelie, P.C., Van Staveren, W.A., Roos, A.H., Tuinstra, L.G. Burema, J:. Nutrients and contaminants in Human milk from mothers on macrobiotic and omnivorous diets. Eur J Clin Nutr, 1992; 46:355- 366.
9. Donangelo, C.M., Trugo, N.M., Koury, J.C., Barreto Silva, M.I., Freitas, L.A., Feldheim, W., Barth, C.: Iron, zinc, folate and vitamin B12 nutritional status and milk composition of low income Brazilian mothers. Eur J Clin Nutr, 1989; 43: 253- 266.
10. Food and Nutrition Board: Vitamin B12 Dietary Reference Intakes: Thiamin, Riboflavin, Niacin, Vitamin B- 6, Vitamin B12, Pantothenic Acid, Biotin, and Choline. National Academy Pres, Washington, 1998.
11. Ford, C., Rendle, M., Tracy, M., Richardson, V., Ford, H.: Vitamin B12 levels in human milk during the first nine months of lactation. Int J Vitam Nutr Res, 1996; 66: 329- 331.
12. Healton, E.B., Savage, D.G., Brust, J.C., Garrett, T.F., Lindenbaum, J.: Neurological aspects of cobalamin deficiency. Medicine, 1991; 70: 229- 244.
13. Herbert, V.: Vitamin B12 in Present Knowledge in Nutrition. 17th ed. International Life Sciences Institute Pres, Washington, 1996.
14. Mills, C.F.: Recent Advances in Animal Nutrition. In: U. HARESIGN, eds. Cobalt deficiency and cobalt requirements of ruminants. Butterworth, London. 129, 1981.
15. Monsen, A.L.B., Ueland, P.M.: Homocysteine and methylmalonic acid in diagnosis and risk assessment from infancy to adolescent. Am J Clin Nutr, 2003; 78: 7- 21.
16. Sawaya, W.N., Khalil, J.K., Al-Shalhat, A.F.: Mineral and vitamin content of goat's milk. J Am Diet Assoc, 1984; 84(4): 433- 435.
17. Shrimpton, D.H., Duckworth, J.: The vitamin-B12 (Cobalamin) content of the milk of lowland and hill sheep. J Sci Food Agr, 2006; 4(6): 301- 304.
18. Smith R, Cobalt M.: Trace Elements in Human and Animal Nutrition. In: W. MERTZ, eds. Academic Pres, San Diego. 143- 184, 1987.
19. Specker, B.L., Black, A., Allen, L., Morrow, F.: Low milk concentrations are related to low serum concentrations in vegetarian women and to methylmalonic aciduria in their infants. Am J Clin Nutr, 1990; 52: 1073- 1076.
20. State Planning Organization-SPO: The specialization comission report of food industry. Sub comission report of milk and milk products industry. 8th Development Plan. DPT-ÖİK, 2636/644, DPT,, Ankara, 2001.
21. State Institute of Statistics-SIS: Statistic yearbook of Turkey. http: //www.die.gov.tr, 2003.
22. Tan, S., Erturk, E.: Süt ve Süt Mamulleri Durum ve Tahmin Raporu, TEAE, Ankara, 2002.
23. Trugo, N.M., Sardinha, F.: Cobalamin and cobalamin-binding capacity in Human milk. Nutr Res, 1994; 14: 22- 33.