Mezbaha materyali inek ovaryumlarindan elde edilen primer oositler 6 gruba ayrıldı ve farklı medyumlarda in vitro olgunlaştırıldı. Grup l:%20 ECS ilaveli MPM (Modifiye Parker) medyumu; Grup 2:%20 ECS ve 10 |ig/ml FSH ilaveli MPM medyumu; Grup 3:%20 ECS ve 50u.g/ml LH ilaveli MPM medyumu;Grup 4:%20ECS ilaveli TCM-199 (Doku Kültür) medyumu; Grup 5: %20 ECS ve 10 ug/ml FSH ilaveli TCM-199 medyumu; Grup 6: %20 ECS ve 50 ug/ml LH ilaveli TCM-199 medyumu.Allı ayrı grupta in vitro olgunlaştırman sığır oositlerinin, BSA, heparin ve PHE ilaveli Ty-rode-laktat medyumunda in vitro fertilizasyonunu takiben gruplara göre sırasıyla %23.3 (7/30), %10.0 (3/30), %3.2 (1/31), 0 (0/33), %39.3 (11/28) ve %38.7 (12/31) fertilizasyon oranları elde edildi, ikinci ve 3. grup arasındaki fark benzer, diğer tüm gruplar arasındaki farklar ise oldukça anlamlı bulundu (pO.001).

Oocytes collected from the ovaries of slaughtered cows were divided into 6 groups and matured in vitro in different media. Group 1: MPM (Modified Parker's) medium sup¬plemented with ECS 20%; Group 2: MPM medium supplemented with ECS 20% and 10 (Xg/ml FSH; Group 3: MPM medium supplemented with ECS 20% and 50 (ig/ml LH; Group 4: TCM-199 (Tissue Culture) medium supplemented with ECS 20%; Group 5: TCM-199 medium supple¬mented with ECS 20% and 10 U-g/ml FSH; Group 6: TCM-199 medium supplemented with ECS 20% and 50 (ig/ml LH. After maturation in 6 different media and fertilization in Tyrode-lactate medium supple¬mented with BSA, heparin and PHE, fertilization rates according to the maturation groups were 23.3% (7/30), 10.0% (3/30), 3.2% (1/31), 0 (0/33), 39.3% (11/28) and 38.7% (12/31), respectively. Although the difference between the second and third groups was not significant statistically, dif¬ferences among all groups were significantly important (p