Saeed NAZIFI, Mehdi FANI MOHEBBI, Abdollah MIRZAEI, Mohammad Reza TABANDEH, Zahra SHABBOOEI

Selenium, Copper, Zinc, Manganese and Their Relevant Antioxidant Enzymes in Plasma of Grazing Pregnant Ewes during Dry Season

Yağlı kuyruklu gebe koyunlarda, hayvanların oksidatif durumunun yanı sıra ilişkili antioksidan enzimlerin aktiviteleri hakkında Bakır (Cu), Çinko (Zn), Mangan (Mn) ve Selenyum'un (Se) plazma seviyeleri ölçüldü. 47 adet koyunun tüm kan örnekleri, 51 günlük üreme periyodlarının ilk gününde (gebelik öncesi) ve 120 gün sonra (gebeliğin 70-120 günleri) alındı. Gebelik, 120 günlük örneklerde plazma progesteronu ölçülerek 43 koyunda doğrulandı. Tüm çalışma periyodunda, koyunlar düşük-orta kaliteli otlaklarda ve ekin anızında otlatıldı ve hayvan başına günlük yaklaşık 1 kg, %40 kaba yonca samanı ve %60 buğday samanı karışımı ile ayrıca en az 100 g arpa tanesi önerildi. İz mineral ilavesi, seçmeksizin uygundu. Plazmada Cu, Zn, Mn ve Se konsantrasyonun yanı sıra eritrositlerde malondialdehit (MDA: lipid peroksidasyonu indeksi), superoksit dismutaz (SOD) ve glutatyon peroksidaz (GPX) düzeyi ölçüldü. MDA seviyesi, oksidatif stresin varlığıyla 120ci günde arttı (P

KURU DÖNEMDE OTLATILAN GEBE KOYUNLARIN PLAZMALARINDA SELENYUM, BAKIR, ÇİNKO, MANGAN VE ONLARLA İLİŞKİLİ ANTİOKSİDAN ENZİMLER

The plasma levels of copper (Cu), zinc (Zn), manganese (Mn) and selenium (Se) were measured in pregnant fat tailed ewes in relation to the activities of the relevant antioxidant enzymes as well as the oxidative status of the animals. Whole blood samples of 47 ewes were taken at the first day of a 51-day breeding period (before pregnancy) and 120 days later (70-120 days of pregnancy). Pregnancy was confirmed in 43 ewes by measuring plasma progesterone in the samples of day 120. For the whole period of the study, the ewes were grazing on medium-to-low quality pastures and cereal stubble and were offered about 1 kg/head/day of a mixture of alfalfa hay (40%) and wheat straw (60%) plus at least 100g barley grain/head/day. A trace mineralized supplement was available free choice. The level of malondialdehyde (MDA; an index of lipid peroxidation) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) were measured in erythrocytes as well as the concentrations of Cu, Zn, Mn and Se in plasma. In the ewes with confirmed pregnancies the results were compared between days 1 and 120. MDA level increased (P

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