Mithat EVECEN, Sema BİRLER, Ramazan ARICI, Serhat PABUCCUOĞLU, Elif ÖZTÜRK KARAMAN, Kamber DEMİR

Koyun Embriyolarının Dondurulmasında Farklı Soğutma Hızlarının Embriyoların Canlılığı veGebelik Oranları Üzerindeki Etkisi

In the first stage of the study, oocytes obtained from ovaries from abbatoir (n=2990) were incubated in maturation medium for 24 h. The maturated oocytes were left for in vitro fertilization (IVF) for 20 hours. The cleavaged embryos (n=1305) were left for in vitro culture (IVC) for six days. Then morula-blastocyst stage embryos divided randomly in three groups (Grup I: 0.5°C/min, Grup II: 0.8°C/min, Grup III: 1°C/min). Embryos in each group (n=50) were frozen at different cooling rates in 1.5 M ethylene glycol containing freezing medium. As a result, 0.5°C/min cooling rate group was found as the most successful group (P

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