Mehmet Erman OR, Lora KOENHEMSİ, Kemal METİNER, Baran ÇELİK, Seyyal AK, Arzu Funda BAĞCIGİL

Examination of Vancomycin Resistant Enterococci (VRE) Isolated from Canine and Feline Rectal Swabs

Kedi ve Köpek Rektal Sıvaplarından İzole Edilen Vankomisin Dirençli Enterokokların (VRE) Araştırılması Bu çalışmanın amacı kedi ve köpeklerde vankomisin dirençli enterokokların (VRE) varlığının araştırılması, fenotipik olarak antimikrobiyal direnç profillerinin incelenmesi ve vankomisin direnci ile ilişkili genlerin dağılımının PCR ile saptanmasıdır. Bu amaçla kedi ve köpeklerden rektal sıvap örnekleri toplandı ve VRE izolasyonu yönünden incelendi. İzolatların identifikasyonunu takiben, Clinical and Laboratory Standards Institute (CLSI) standartlarına göre antimikrobiyal duyarlılıkları araştırıldı. Son olarak, vankomisin dirençle ilişkili genleri; vanA, vanB, vanC1 ve vanC2/C3 gen bölgeleri PCR ile saptandı. Toplam 157 (86 köpek, 71 kedi) sıvap örneği incelendi %12,7' sinden VRE izole edildi. 12 köpek ve 8 kedi örneğinden VRE izole edildi. Antibiyotik duyarlılık sonuçları izolatlar arasında farklılıklar göstermekle birlikte tüm izolatlar arasında enrofloksasin direnci en sık karşılaşılan dirençti. Aynı zamanda çoklu antibiyotik direnci de saptandı. Sonuç olarak, pet hayvanlarında VRE dağılımının sürekli taranması, antibiyotik direnci ile ilişkili olası halk sağlığı problemlerinin önlenmesi açısından önemi vurgulandı.

Kedi ve Köpek Rektal Sıvaplarından İzole Edilen Vankomisin Dirençli Enterokokların (VRE) Araştırılması

The aim of this study was to determine the occurrence of vancomycin resistant enterococci (VRE) in dogs and cats, examine the antimicrobial resistance profiles phenotypically, and the distribution of the vancomycin resistance associated genes by PCR. For this purpose, rectal swabs from animals were collected and processed for VRE isolation. After the identification of the isolates, antimicrobial susceptibility of the isolates were determined according to the Clinical and Laboratory Standards Institute (CLSI) standards. Finally, distribution of the vancomycin resistance associated genes; vanA, vanB, vanC1 and vanC2/C3 were determined by PCR. Totally 157 (86 canine, 71 feline) rectal swab samples were examined. VRE were isolated from 12 canine and 8 feline samples. The VRE isolation rate in companion animals was 12.7%. Antimicrobial susceptibility results varied among the isolates; however, enrofloxacin resistance was common in both species. Multidrug resistance was also detected. As a conclusion, screening of distribution of VRE among pet animals would be useful to detect any emerging antimicrobial resistance problem related to public health.

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Bağcigil, A.F., İkiz, S., Güzel, O., Parkan-Yaramış, Ç., Ilgaz, A., 2012. Species distribution of methicillin resistant staphylococci isolated from animals, environmental samples and staff. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 38, 151-160.
Bağcıgil, A.F, İkiz, S., Ak, S., Özgür, N.Y., 2015. Isolation of vancomycin resistant enterococci from animal faeces, detection of antimicrobial resistance profiles and vancomycin resistant genes. Kafkas Universitesi Veteriner Fakültesi Dergisi 21, 87-94.
Boynukara, B., Ekin, İ.H., Aksakal, A., Gülhan, T., 2002. Isolation and antibiotic susceptiblity of enterococci from human, dog and cat faeces. Veteriner Hekimleri Mikrobiyoloji Dergisi 2, 37-42.
Clinical and Laboratory Standards Institute, 2006. Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. CLSI document M100-S16 [ISBN 1-56238-588-7). Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA.
Coombs, G.W., Kay, I.D., Steven, R.A., Pearman, J.W., Bertolatti, D., Grubb, W.B., 1999. Should genotyping testing be done on all phenotypically vancomycin- resistant enterococci detected in hospitals? Journal of Clinical Microbiology 37, 1229-1230.
Corso, A., Faccone, D., Gagetti, P., Togneri, A., Lopardo, H., Melano, R., Rodriguez, V., Rodriguez, M., Galas, M., 2005. First report of VanA Enterococcus gallinarum dissemination within an intensive care unit in Argentina. International Journal of Antimicrobial 25, 51-56. de Leener, E., Decostre, A., de Graef, E.M., Moyaert, H., Haesebrouck, F., 2005. Presence and mechanism of antimicrobial resistance among enterococci from cats and dogs. Microbial Drug Resistance 11, 395-402.
Elsayed, S., Hamilton, N., Boyd, D., Mulvey, M., 2001. Improved primer design for multiplex PCR analysis of vancomycin-resistant Enterococcus spp. Journal of Clinical Microbiology 39, 2367- 2368.
Ghosh, A., KuKanich, K., Brown, C.E., Zurek, L., 2012. Resident cats in small animal veterinary hospitals carry multi- drug resistant enterococci and are likely involved in cross-contamination of the hospital environment. Frontiers in Microbiololgy 3, 1-14.
Herrero, I.A., Fernandez-Garayzabal, J.F., Moreno, M.A., Dominguez, L., 2004. Dogs should be included in surveillance programs for vancomycin-resistant enterococci. Journal of Clinical Microbiology 42, 1384- 1385.
Hershberger, E., Oprea, S.F., Donabedian, S.M., Perri, M., Bozigar, P., Bartlett, P., Zervos, M.J., 2005. Epidemiology of antimicrobial resistance in enterococci of animal origin. Journal of Antimicrobial Chemotherapy 55, 127-130.
Jackson, C.R., Fedorka-Cray, P.J., Davis, J.A., Barrett, J.B., Frye, J.G., 2009. Prevalence, species distribution and antimicrobail resistance of enterococci isolated from dogs and cats in the United States. Journal of Applied Microbiology 107, 1269-1278.
Kariyama, R., Mitsuhata, R., Chow, J.W., Clewell, D.B., Kumon, H., 2000. Simple and reliable Multiplex-PCR assay for surveliance isolates of vancomycin-resistant enterococci. Journal of Clinical Microbiology 38, 3092- 3095.
Ke, D., Picard, F.J., Martineau, F., Ménard, C., Roy, P.H., Ouellette, M., Bergeron, M. G., 1999. Development of a PCR aasay for rapid detection of enterococci. Journal of Clinical Microbiology 37, 3497-3503.
Lopez, M., Tenorio, C., Torres, C., 2013. Study of vancomycin resistance in faecal enterococci from healthy humans and dogs in Spain a decade after the avoparcin ban in Europe. Zoonoses and Public Health 60,160-167.
NCCLS, 2004. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals; Informational Supplement. NCCLS document M31-S1 (ISBN 1-56238-534-8). NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898, USA.
Sayıner, H.S., 2008. Hastanemizde sürveyansla saptanan VRE'lerin dağılımı, antibiyotiklere duyarlılıkları ve kolonize hastalarda risk faktörlerinin değerlendirilmesi, Yüksek Lisans Tezi, Okmeydanı Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Kliniği, İstanbul. Hastalıkları ve Klinik
Türkyılmaz, S., Erdem, V., Bozdoğan, B. 2010. Investigation of antimicrobial susceptibility for enterococci isolated from cats and dogs and the determination of resistance genes by polymerase chain reaction. Turkish Journal of Veterinary and Animal Science 34, 61-68.
Weese, J.S., 2008. Antimicrobial resistance in companion animals. Animal Health Research Reviews 9, 169-176.