Makbule AYDIN, Şule SEÇKİN, Şeyda ALSANCAK, Başaran Canan KÜÇÜKERGİN

Effect OFD-Galactosamine on oxidative stress and apoptosis in the laver of rats

Amaç: Çalışmamızda, yüksek doz D-galaktozamin (D-GalN) uygulamasının sıçanlarda oksidatif stres ve apoptoz üzerine etkisini inceledik. Gereç ve yöntem: Sıçanlara intraperitonal olarak, tek dozda 1g/kg D-GalN uygulandı. Sıçanların karaciğer homojenatlarında malondialdehit(MDA) ve glutatyon (GSH) düzeyleri spektrofotometrik yöntemle ölçüldü. Karaciğer dokusunda TNF- alpha düzeylerinin ölçülmesi için EL‹SA yöntemi uygulandı. Apoptozun bir göstergesi olan DNA fragmantasyonu kalitatif olarak agoroz jel elektroforezi ile gözlendi.Bulgular: Lipid peroksidasyonun bir göstergesi olan MDA düzeyleri 1g/kg başına D-GalN uygulamasından 24 saat sonra anlamlı olarak artış gösterirken GSH düzeylerinde azalma saptandı. Hepatik TNF- alpha düzeyleri ise kontrol gruba göre anlamlı olarak azaldı. DNA fragmantasyonu jel elektroforezi ile yapılan incelemede gözlenmedi.Sonuç: Yüksek doz GalN uygulamasının oksidatif stresi indüklediği ama apoptoz üzerinde etkili olmadığı ileri sürülebilir.

Sıçanların karaciğerlerinde D-Galaktozaminin oksidatif stres ve apoptoz üzerine etkisi

Objective: This study was done to clarify the effect of high dose of D-Galactosamine (GalN) on oxidative stress and apoptosis in experimental rats. Materials and methods: A single dose of 1 g/kg D-GalN dissolved in 0.9% NaCl was given to rats intraperitoneally. Malondialdehyde (MDA) and glutathione (GSH) levels in liver homogenates were measured spectrophotometrically. TNF-α concentration in liver was determined by enzyme linked immunosorbent assay (ELISA). DNA fragmentation which is considered to be diagnosis of apoptosis was observed by agarose gel electrophoresis.Results: MDAlevel which is used as an indicator of lipid peroxidation, increased significantly, 24hr after intraperitoneal administration of D-GalN (1g/kg body weight) to rats. GSH, the endogenous antioxidant, was found decreased in D-GalN treated rats compared to control rats. Hepatic TNF-α level decreased significantly compared with that in control group. No DNA fragmentation was observed by gel electrophoresis.Conclusion: These results suggest that high dose of D-GalN induces oxidative stress but not apoptosis in the liver of experimental rats.

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