Neslihan YILDIRIM, Dilek K. SELÇUKİ, Pervin VURAL, Mukaddes CANBAZ

Acne vulgarisli olgularda plazma nitratı nitrit düzeylerinin incelenmesi

Amaç: Akne açık ve kapalı komedon oluşumu, eritemli papül ve püstüller, nodüller, psödokistler ve bazen de nedbe oluşumu ile karakterizedir. Acne vulgaris etyopatogenezinde sebum salınımında artış, duktal hiperkeratinizasyon (hiperkornifikasyon), foliküllere Propionibacterium Acnes kolonizasyonu ve inflamasyonun olduğu düşünülmektedir. Nitrik oksit (NO), derinin keratinosit, fibroblast, endotel, Langerhans ve mast hücrelerinde bulunan nitrik oksid sentaz (NOS) tarafından oluşturulan bir inorganik moleküldür. Sitokinlerin etkisi ile keratinosit ve mast hücrelerinde NOS indüklenmektedir. Oluşan NO guanilat siklazı stimüle ederek keratinosit ve mast hücrelerinde mitojenik etkili olan cGMP düzeylerinin artmasına ve böylece hiperkeratinizasyona neden olmaktadır. Bu çalışmanın amacı nitrik oksit düzeylerinin acne vulgarisin etyopatogenezindeki rolünü incelemekti. Gereç ve yöntem: Bu çalışmaya Acne Vulgaris tanısı konmuş 30 hasta ile, yapılan sistemik muayeneleri sonucu sağlıklı oldukları saptanan 16 kişi kontrol grubu olarak alındı. Gerek deney, gerekse kontrol grubunu oluşturan olgulardan sabah aç karına alınan venöz kan örneklerinde NO’in metabolik ürünleri olan nitrat/nitrit (NOx) düzeylerine Griess reaksiyonu ile bakıldı. Elde edilen sonuçlar Student’s t testi kullanılarak kıyaslandı. Bulgular: Deney grubunda nitrat/nitrit düzeylerinde kontrol grubuna göre istatistiki yönden anlamlı (p< 0,05) bir artış gözlendi. Sonuç: Sonuç olarak, akneli olgulardaki plazma nitrat/nitrit düzeylerindeki artışın bu bileşiklerin üretiminin hızlanması na bağlı olduğu söylenebilir.

Evaluation of plasma nitrate nitrite levels in patients with acne vulgaris

Objective:Acne is characterized by open and close comedon, erythematous papules, pustules, pseudocysts, nodules and scar formation. Factors playing role at the etiopathogenesis include increased sebum secretion, ductal hyperkeratinization (hypercornification), Propionibacterium Acnes colonization at the follicules and inflammation. Nitric oxide (NO) is an inorganic molecule which is synthesized by NO synthetase (NOS) found in keratinocyte, fibroblast, endothel, Langerhans and mast cells of the skin. NOS found in the keratinocytes and mast cells is induced by cytokines. Produced NO stimulates guanylate cyclase which increases cGMP levels; cGMP has mitogenic effects on keratinocytes and mast cells, therefore causes hyperkeratinization. The aim of this study was to evaluate the role of nitric oxide levels in the etiopathogenesis of acne vulgaris. Materials and methods: Study group was composed of 30 patients diagnosed as acne vulgaris and 16 healthy subjects were included as the control group. Blood was taken early in the morning at fasting from both groups. The metabolic products of NO - plasma nitrite/nitrate were measured by Griess reaction. Statistical evaluation of the data was performed using the Student’s t test. Results: Plasma nitrite/nitrate levels of the study group were significantly elevated in patients with acne vulgaris when compared to the controls (p

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