Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei

With a cosmopolite widespread worldwide, Ascocyhta rabiei may result in yield losses in chickpea fields up to 100% when the climate conditions were available for growth and development of the pathogen. Effects of culture media, temperature, pH and light regimes on mycelial growth of nine A. rabiei isolates isolated from different agroecological zones of Yozgat province (Turkey) were investigated in this study. It was observed that culture media, temperature, pH and light regimes significantly influenced mycelial growth rates of A. rabiei isolates. A. rabiei isolated developed smooth, sparse and immersed mycelium in culture media. Mycelium was characterized with pale cream, light greenish grey or grey-black color. Malt Extract Agar (MEA) medium was identified as the best culture medium promoting mycelial growth; the temperature required for maximum daily mycelial growth (2.0 mm day-1) was identified as 20.0°C. Present isolates formed smaller-diameter colonies at acidic pH values as compared to basic pH values. The pH values of 6.0 and 7.0 promoted mycelial growth. Light significantly influenced radial mycelial growth of YBUAr7 isolate of A. rabiei. Prolonged light exposures promoted radial growth in culture media and the cultures incubated under continuous light exhibited greater radial growth rates than the cultures incubated under 12 h photoperiods and 24 h dark.

Keywords:

Ascocyhta rabiei, Environmental factors Growth,

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