Postharvest mycobial contaminants of white button mushroom (Agaricus bisporus) and their management using plant essential oils

Anahtar Kelimeler:

Aspergillus niger, Botanicals, White button mushroom, Fungal contaminants, Fungitoxic effect

Postharvest mycobial contaminants of white button mushroom (Agaricus bisporus) and their management using plant essential oils

Being highly perishable, mushrooms’ quality and shelf life is affected by various factors during postharvest conditions, among which fungal contamination is the main cause. The goal of this study is to identify and manage fungal contaminants present in mushrooms during postharvest conditions. A total of 23 fungi were isolated as contaminants from the samples of Agaricus bisporus collected from three major vegetable markets in Kathmandu city, Nepal. Aspergillus niger, Aspergillus flavus, and Rhizopus stolonifer were found to be the most frequent fungal contaminants. These were treated with various concentrations of essential oils (EOs) of Cinnamomum tamala, Mentha spicata, Zanthoxylum armatum, and Eucalyptus citriodora using poisoned food technique. Significant (p<0.05) inhibition of mycelial growth and spore germination was found in all testedfungi by all EOs. A strong inhibitory action of M. spicata oil was recorded against A. flavus and R. stolonifer while, A. niger was best controlled by C. tamala oil at the concentration of 20 µl/ml. These results suggest that EOs of three tested plants could be a good alternative to control fungal contaminants and extend the shelf life of Agaricus bisporus in postharvest conditions.

Keywords:

Aspergillus niger, Botanicals, White button mushroom, Fungal contaminants, Fungitoxic effect,

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Adhikari, H.S., & Jha, S.K. (2017). Postharvest microbial contamination in oyster mushroom and their management using plant Essential oils. Bio Bulletin, 3(1), 104-108. http://www.biobulletin.com/
Adhikari, H. S., & Jha, S. K. (2020). Postharvest Loss and Mycofloral Association in Some Culinary Mushrooms in Kathmandu City, Nepal. Amrit Research Journal, 1(1), 13-19. https://doi.org/10.3126/arj.v1i1.32448
Barbosa, L. C. A., Filomeno, C. A., & Teixeira, R. R. (2016). Chemical variability and biological activities of Eucalyptus spp. essential oils. Molecules, 21(12), 1671. https://doi.org/10.3390/molecules21121671
Barnett, H. L., & Hunter, B. B. (1972). Illustrated genera of imperfect fungi. Illustrated genera of imperfect fungi., (3rd ed).
Bassolé, I. H. N., & Juliani, H. R. (2012). Essential oils in combination and their antimicrobial properties. Molecules, 17(4), 3989-4006. https://doi.org/10.3390/molecules17043989
Biswas, M.K. (2014). Microbial contaminants in Oyster mushroom (Pleurotus ostreatus) cultivation their management and role of meteorological factors. In Proceedings of the 8th International Conference on Mushroom Biology and Mushroom Products (ICMBMP8), 567-575.
Bluma, R., Amaiden, M. R., Daghero, J., & Etcheverry, M. (2008). Control of Aspergillus section Flavi growth and aflatoxin accumulation by plant essential oils. Journal of applied microbiology, 105(1), 203-214. https://doi.org/10.1111/j.1365-2672.2008.03741.x
Caccioni, D. R., & Guizzardi, M. (1994). Inhibition of germination and growth of fruit and vegetable postharvest pathogenic fungi by essential oil components. Journal of Essential Oil Research, 6(2), 173-179. https://doi.org/10.1080/10412905.1994.9698349
Dandge, V. S. (2012). Studies on some fungi in button mushroom cultivated in Akola (Amanatpur). Recent Research in Science and Technology, 4(10), 28-30. http://recent-science.com/
Edris, A. E. (2007). Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 21(4), 308-323. https://doi.org/10.1002/ptr.2072
Falleh, H., Jemaa, M. B., Saada, M., & Ksouri, R. (2020). Essential oils: A promising eco-friendly food preservative. Food Chemistry, 330, 127268. https://doi.org/10.1016/j.foodchem.2020.127268
FAO. (2015). Global Initiative on Food Loss and Waste Reduction. Food and Agriculture Organization of the United Nations. SAVE FOOD. [Online]. Available: http://www.fao.org/3/ai4068e.pdf
Farzaneh, M., Kiani, H., Sharifi, R., Reisi, M., & Hadian, J. (2015). Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest Biology and Technology, 109, 145-151. https://doi.org/10.1016/j.postharvbio.2015.06.014
Ghalem, B.R. (2016). Essential oils as antimicrobial agents against some important plant pathogenic bacteria and fungi. In Plant-Microbe Interaction: An Approach to Sustainable Agriculture, Springer, Singapore, 271-296. https://doi.org/10.1007/978-981-10-2854-0_13
Gomes, C., Moreira, R.G., & Castell‐Perez, E. (2011). Poly (DL‐lactide‐co‐glycolide)(PLGA) nanoparticles with entrapped trans‐cinnamaldehyde and eugenol for antimicrobial delivery applications. Journal of Food Science, 76(2), N16-N24. https://doi.org/10.1111/j.1750-3841.2010.01985.x
Grover, R. K., & Moore, J. D. (1962). Toximetric studies of fungicides against brown rot organism Sclerotina fruticola. Phytopathology, 52, 876-880.
Guerra, I.C.D., de Oliveira, P.D.L., de Souza Pontes, A.L., Lúcio, A.S.S.C., Tavares, J.F., Barbosa-Filho, J.M., Madruga, M.S., & de Souza, E.L. (2015). Coatings comprising chitosan and Mentha piperita L. or Mentha× villosa Huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit. International Journal of Food Microbiology, 214, 168-178. https://doi.org/10.1016/j.ijfoodmicro.2015.08.009
Haddi, K., Faroni, L. R., & Oliveira, E.E. (2017). Cinnamon Oil, In: Green Pesticides Handbook, Essential Oils for Pest Control. 1st Edition. Boca Raton: CRC Press, 523, 117-150. https://doi.org/10.1201/9781315153131
Hodges, R. J., Buzby, J. C., & Bennett, B. (2011). Postharvest losses and waste in developed and less developed countries: opportunities to improve resource use. The Journal of Agricultural Science, 149(S1), 37-45. https://doi.org/10.1017/S0021859610000936
Hu, F., Tu, X. F., Thakur, K., Hu, F., Li, X. L., Zhang, Y. S., Zhang, J. G., & Wei, Z. J. (2019). Comparison of antifungal activity of essential oils from different plants against three fungi. Food and Chemical Toxicology, 134, 110821. https://doi.org/10.1016/j.fct.2019.110821
Humble, S., & Reneby, A. (2014) Post-harvest losses in fruit supply chains: a case study of mango and avocado in Ethiopia. Second cycle, A2E. Uppsala: SLU, Dept. of Economics. https://stud.epsilon.slu.se/7521/
Hyldgaard, M., Mygind, T., & Meyer, R. L. (2012). Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Frontiers in microbiology, 3, 12. https://doi.org/10.3389/fmicb.2012.00012
Ju, J., Xie, Y., Guo, Y., Cheng, Y., Qian, H., & Yao, W. (2019). Application of edible coating with essential oil in food preservation. Critical Reviews in Food Science and Nutrition, 59(15), 2467-2480. https://doi.org/10.1080/10408398.2018.1456402
Kalemba, D. A. A. K., & Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Current medicinal chemistry, 10(10), 813-829. https://doi.org/10.2174/0929867033457719
Kedia, A., Dwivedy, A. K., Jha, D. K., & Dubey, N. K. (2016). Efficacy of Mentha spicata essential oil in suppression of Aspergillus flavus and aflatoxin contamination in chickpea with particular emphasis to mode of antifungal action. Protoplasma, 253(3), 647-653. https://doi.org/10.1007/s00709-015-0871-9
Kertesz, M. A., & Thai, M. (2018). Compost bacteria and fungi that influence growth and development of Agaricus bisporus and other commercial mushrooms. Applied microbiology and biotechnology, 102(4), 1639-1650. https://doi.org/10.1007/s00253-018-8777-z
Király, Z., Klement, Z., Solymosy, F., & Voros, J. (1974). Methods in plant pathology with special reference to breeding for disease resistance.
Mishra, R. (2022). Problems of Fungal Contaminants and Cultivation Strategies of Certain Medicinal Mushrooms. In Biology, Cultivation and Applications of Mushrooms, Springer, Singapore, 611-627. https://doi.org/10.1007/978-981-16-6257-7_24
Nazzaro, F., Fratianni, F., Coppola, R., & De Feo, V. (2017). Essential oils and antifungal activity. Pharmaceuticals, 10(4), 86. https://doi.org/10.3390/ph10040086
Omotayo, O. P., Omotayo, A. O., Mwanza, M., & Babalola, O. O. (2019). Prevalence of mycotoxins and their consequences on human health. Toxicological Research, 35(1), 1-7. https://doi.org/10.5487/TR.2019.35.1.001
Ons, L., Bylemans, D., Thevissen, K., & Cammue, B. P. (2020). Combining biocontrol agents with chemical fungicides for integrated plant fungal disease control. Microorganisms, 8(12), 1930. https://doi.org/10.3390/microorganisms8121930
Piras, A., Porcedda, S., Falconieri, D., Maxia, A., Gonçalves, M. J., Cavaleiro, C., & Salgueiro, L. (2021). Antifungal activity of essential oil from Mentha spicata L. and Mentha pulegium L. growing wild in Sardinia island (Italy). Natural Product Research, 35(6), 993-999. https://doi.org/10.1080/14786419.2019.1610755
Prakash, B., Kedia, A., Mishra, P. K., & Dubey, N. K. (2015). Plant essential oils as food preservatives to control moulds, mycotoxin contamination and oxidative deterioration of agri-food commodities–Potentials and challenges. Food Control, 47, 381-391. https://doi.org/10.1016/j.foodcont.2014.07.023
Prakash, B., Singh, P., Kedia, A., & Dubey, N. K. (2012). Assessment of some essential oils as food preservatives based on antifungal, antiaflatoxin, antioxidant activities and in vivo efficacy in food system. Food Research International, 49(1), 201-208. https://doi.org/10.1016/j.foodres.2012.08.020
Ramos, M., Burgos, N., Barnard, A., Evans, G., Preece, J., Graz, M., Ruthes, A.C., Jiménez-Quero, A., Martínez-Abad, A., Vilaplana, F. and Ngoc, L.P. (2019). Agaricus bisporus and its by-products as a source of valuable extracts and bioactive compounds. Food chemistry, 292, 176-187. https://doi.org/10.1016/j.foodchem.2019.04.035
Raut, J.K. (2013). “Adhunik chyau kheti prabidhi” (=Modern Mushroom Cultivation Technology) 1st ed. Heritage Publisher and Distributor Pvt. Ltd. Kathmandu, Nepal, 10–15
Raut, J. K. (2019). Current status, challenges and prospects of mushroom industry in Nepal. International Journal of Agricultural Economics, 4(4), 154-160. https://doi.org/10.11648/j.ijae.20190404.13
Royse, D. J., Baars, J., & Tan, Q. (2017). Current overview of mushroom production in the world. Edible and medicinal mushrooms: technology and applications, 5-13. https://doi.org/10.1002/9781119149446.ch2
Sharma, R. R., Singh, D., & Singh, R. (2009). Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biological Control, 50(3), 205-221. https://doi.org/10.1016/j.biocontrol.2009.05.001
Shrestha, U. B. (2014). Chasing interconnections: Mountains, mushrooms and people. University of Massachusetts Boston. https://scholarworks.umb.edu/doctoral_dissertations/154/
Sovová, H., & Aleksovski, S. A. (2006). Mathematical model for hydrodistillation of essential oils. Flavour and fragrance journal, 21(6), 881-889. https://doi.org/10.1002/ffj.1729
Teia, F. K. F., Osman, A., & Osman, M. A. (2018). Preliminary Antifungal Study of Some Essential Oils of Three Medicinal Plants Against Fusarium oxysporum f. sp. ciceris, and Aspergillus Niger. International Journal of Bioorganic Chemistry, 3(1), 1-5. https://doi.org/10.11648/j.ijbc.20180301.11
Thakur, A.K., Singh, R., Gehlot, A., Kaviti, A.K., Aseer, R., Suraparajud, S.K., Natarajand, S.K., & Sikarwar, V.S. (2022). Advancements in solar technologies for sustainable development of agricultural sector in India: a comprehensive review on challenges and opportunities. Environmental Science and Pollution Research, 1-28. https://doi.org/10.1007/s11356-022-20133-0
Thompson, D. P. (1986). Effect of essential oils on spore germination of Rhizopus, Mucor and Aspergillus species. Mycologia, 78(3), 482-485. https://doi.org/10.1080/00275514.1986.12025274
Usman, M., Murtaza, G., & Ditta, A. (2021). Nutritional, medicinal, and cosmetic value of bioactive compounds in button mushroom (Agaricus bisporus): a review. Applied Sciences, 11(13), 5943. https://doi.org/10.3390/app11135943
Wang, X., Gu, S., Chen, B., Huang, J., & Xing, J. (2017). Effect of postharvest L-arginine or cholesterol treatment on the quality of green asparagus (Asparagus officinalis L.) spears during low temperature storage. Scientia Horticulturae, 225, 788-794. https://doi.org/10.1016/j.scienta.2017.07.058
Wang, L., Hu, Q., Pei, F., Mugambi, M. A., & Yang, W. (2020). Detection and identification of fungal growth on freeze‐dried Agaricus bisporus using spectra and olfactory sensors. Journal of the Science of Food and Agriculture, 100(7), 3136-3146. https://doi.org/10.1002/jsfa.10348
Watanabe, T. (2010). Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. CRC press.
Xiang, F., Zhao, Q., Zhao, K., Pei, H., & Tao, F. (2020). The efficacy of composite essential oils against aflatoxigenic fungus Aspergillus flavus in maize. Toxins, 12(9), 562. https://doi.org/10.3390/toxins12090562
Zaidi, S., & Dahiya, P. (2015). In vitro antimicrobial activity, phytochemical analysis and total phenolic content of essential oil from Mentha spicata and Mentha piperita. International Food Research Journal, 22(6), 2440. http://www.ifrj.upm.edu.my/