Ebru BATI AY, Şevket Metin KARA, Muhammed Akif AÇIKGÖZ

Effect of phosphorus fertilization on phenolic compounds and antioxidant activity in Galanthus elwesii Hook.

Snowdrop is a genus of high medicinal value with alkaloids such as galantamine, and lycorine of the Amaryllidaceae family. The present study was conducted to have an effect on the effects of phosphorus (P) treatment on antioxidant activity and phenolic compounds in Galanthus elwesii Hook. The plants were exposed to different concentrations of P ( 0, 3, 6, and 12 kg da-1). The study was carried out in the 2018-2019 growing season. G. elwesii were harvested based on different growing stages (flowering and fruit ripening). In this study, the bulb and roots of the plant were used. Total flavonoid content (TFC), total phenolic content (TPC), phenolic compounds, and antioxidant activity were determined in harvested bulb and roots. The highest TPC was detected as 358.36 mg GAE/g in the flowering period of the plant, and the lowest TPC determined as 80.13 mg GAE/g in the fruit ripening period in the treatment P 6 kg da-1. The highest TFC was detected as 108.07 mg QE/g with the flowering period of the plant, and the lowest TFC was determined as 52.33 mg QE/g in the fruit ripening period in the treatment P 6 kg da-1. The main phenolic component of G. elwesii was determined to be gallic acid (GA). In antioxidant activity, while the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (72.30%) was in the flowering period in the treatment P 6 kg da-1, the highest ferrous ions chelating activity (66.77%) was detected in the fruit ripening period in the treatment P 6 kg da-1. As a result, it was determined that TPC, TFC and DPPH activity in G. elwesii of flowering period ˃fruit ripening period.

Keywords:

Alkaloids bulbous plants, growth and development periods, Galanthus elwesii,

PDF

___

Abbas, G., Chen, Y., Khan, F.Y., Feng, Y., Palta, J.A., Siddique, K.H., (2018). Salinity and low phosphorus differentially affect shoot and root traits in two wheat cultivars with contrasting tolerance to salt. Agronomy 8 (8), 155. https://doi.org/10.3390/agronomy8080155
Albayrak, B., & Elmacı, Ö. L., (2017). Azot ve Kükürtlü Gübre Uygulamalarının Kuru Soğanda (Allium cepa L.) C Vitamini, Antioksidan Aktivite ve Toplam Fenolik Madde Miktarlarına Etkileri. Adnan Menderes üniversitesi Ziraat Dergisi, 14 (2):1-5.
Ay, B. E., Gül, M., Açikgöz, M.A., Yarilgaç, T., & Kara, Ş. M. (2018). Assessment of Antioxidant Activity of Giant Snowdrop (Galanthus elwesii Hook) Extracts with Their Total Phenol and Flavonoid Contents. Indian Journal of Pharmaceutical Education and Research, 52(Issue 4 (Suppl), 128-132.
Batı Ay, E. (2019). Farklı Fosfor ve Çinko Dozları Uygulanan Kardelende (Galanthus elwesii Hook.) Fenolik Bileşikler, Alkaloit İçeriği ve Antioksidan Aktivitenin Bitki Organlarına ve Gelişme Dönemlerine Göre Değişimi.
Brand-Williams W, Cuvelier ME, Berset C, (1995). Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Sci. Techno., 28, 25–30.
Ay, E. B., Açıkgöz, M. A., Kocaman, B., Mesci, S., Kocaman, B., Yıldırım, T. (2023). Zinc and phosphorus fertilization in Galanthus elwesii Hook: Changes in the total alkaloid, flavonoid, and phenolic content, and evaluation of anti-cancer, anti-microbial, and antioxidant activities. Scientia Horticulturae, 317, 112034.
Aydın, Ç. (2016). Bazı geofit ekstraktlarının farklı biyolojik aktivitelerinin araştırılması. Doktora Tezi, Pamukkale Üniversitesi, Fen Bilimleri Enstitüsü, Denizli.
Deniz, N. (2016). Crocus cancellatus Herbert subsp. mazziaricus (Herbert) Mathew ve Crocus pallasii Goldb. subsp. pallasii Goldb. taksonları ekstraklarının aktif bileşenleri ve bazı biyolojik aktivitelerinin belirlenmesi. Yüksek Lisans Tezi, Pamukkale Üniversitesi, Fen Bilimleri Enstitüsü, Denizli.
Deveci, E., Tel-Çayan, G., Duru, M. E. (2018). Phenolic profile, antioxidant, anticholinesterase, and anti-tyrosinase activities of the various extracts of Ferula elaeochytris and Sideritis stricta. International journal of food properties, 21(1), 771-783.
Erenler, R., Genç, N, Elmastaş, M., Eminağaoğlu, Ö. (2019). Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae). The Turkish Journal of Biodiversity, 2(1), 13-17.
Fernández-López, J., Botella-Martínez, C., Navarro-Rodríguez de Vera, C., Sayas-Barberá, M. E., Viuda-Martos, M., Sánchez-Zapata, E., Pérez-Álvarez, J. A. (2020). Vegetable soups and creams: Raw materials, processing, health benefits, and innovation trends. Plants, 9(12), 1769
Ghane, S. G., Attar, U. A., Yadav, P. B., Lekhak, M. M. (2018). Antioxidant, anti-diabetic, acetylcholinesterase inhibitory potential and estimation of alkaloids (lycorine and galanthamine) from Crinum species: An important source of anticancer and anti-Alzheimer drug. Industrial Crops and Products, 125, 168-177.
Kalayu, G., 2019. Phosphate solubilizing microorganisms: promising approach as biofertilizers. Int. J. Agron. 2019, 1–7. https://doi.org/10.1155/2019/4917256
Kang, E.L., Biscaro, B., Piazza, F., Tesco, G., (2012). BACE1 protein endocytosis and trafficking are differentially regulated by ubiquitination at lysine 501 and the Di- leucine motif in the carboxyl terminus. J. Biol. Chem. 287 (51), 42867–42880. https://doi.org/10.1074/jbc.M112.407072
Korcan, S. E., Bulduk, İ., Kahraman, T., Kayhan, R., Çitekçi, K., Kölemek., H., Öztürk, M. (2018). Narcissus papyraceus Soğanlarında Toplam Fenolik Bileşikler, Toplam Antioksidan Kapasite, Toplam Flavonoid Maddelerin ve Antimikrobiyal Aktivitenin Belirlenmesi. Uşak Üniversitesi Fen ve DoğaBilimleri Dergisi, 2018(1), 10-17.
Orme˜no, E., Fernandez, C., 2012. Effect of soil nutrient on production and diversity of volatile terpenoids from plants. Curr. Bioact. Compd. 8 (1), 71–79. https://doi.org/ 10.2174/157340712799828188
Taiz L. and E. Zeiger. 2010. Plant Physiology fifth Edition. Sinauer Associates., Inc. 23 Plum tree Road, Sunderland, MA 01375, USA
Prakash D., Singh B.N., Upadhyay G., (2007). Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chemistry, 102, 1389–1393.Belirlenmesi. Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi, 2018(1), 10-17.
Pesaresi, A., Lamba, D., Vezenkov, L., Tsekova, D., Lozanov, V., (2022). Kinetic and structural studies on the inhibition of acetylcholinesterase and butyrylcholinesterase by a series of multitarget-directed galantamine-peptide derivatives. Chem. Biol. Interact. 365, 110092 https://doi.org/10.1016/j.cbi.2022.110092
Semerdjieva, I., Sidjimova, B., Yankova-Tsvetkova, E., Kostova, M., Zheljazkov, V.D., (2019). Study on Galanthus species in the Bulgarian flora. Heliyon 5, e03021. https://doi.org/10.1016/j.heliyon.2019
Turan, M. (2016). Cyclamen alpinum ve Cyclamen parviflorum ekstraktlarının fenolik bileşenleri ve bazı biyolojik özelliklerinin belirlenmesi. Yüksek Lisans Tezi, Pamukkale Üniversitesi, Fen Bilimleri Enstitüsü, Denizli.