Erdem TEZCAN, Metin YÜKSEK, Onur YOLAY, Derya SALTIK, Erkan İŞGÖREN

Benzalkonyum Klorür Katkili Pva/Gelatin Nanolif Yapilarinin Antibakteriyel Özellikleri

Poli (vinil alkol) (PVA), iyi kimyasal dayanımı, iyi termal kararlılığı, iyi fiziksel özellikleri, mükemmel biyouyumluluğu ve ucuzluğu nedeniyle çok dikkat çeken hidrofilik, yarı kristalli bir polimerdir. Jelatin (G) biyomedikal uygulamalar için ilginç ve doğal bir polimerdir. Elektroeğirme, yüksek yüzey alanına sahip çok gözenekli nano elyaf üretimi sağlayan basit bir yöntemdir. Elektrospinning yöntemi kullanılarak biyomedikal, filtrasyon, enerji depolama ve koruyucu malzemeler üretmek mümkündür. Benzalkonyum klorür (BAC), ticari yara sargısında yaygın olarak kullanılan ve Gram-pozitif bakterilere karşı güçlü bir role sahip olan bir tür antimikrobiyal katyonik yüzey aktif malzemedir. Bu çalışmada, çeşitli konsantrasyonlarda BAC, PVA ve G içeren çözeltilerin nano lifler üretilmiştir. Elde edilen nano lif yapısının lifleri homojen, sürekli ve yoğundur. İyi mekanik ve antibakteriyel özellikler açısından optimum parametreler belirlenmiştir. 11.63% PVA + 1.0% G +1.0% BAC içeren S2 örneği en iyi morfolojik ve mekanik özellikler göstermesinin yanında en ince elyaf çapına (51 ± 13nm) ve en yüksek dikey dayanıma (4.299MPa) ve yatay dayanıma (4.058MPa) sahiptir. Ayrıca test edilen tüm bakterilere (E. coli, P. aeruginosa, B. subtilis ve S. aureus) karşı antibakteriyel aktiviteye sahiptir. İyi mekanik ve antibakteriyel özelliklere sahip olması nedeniyle, S2 örneğinin tıbbi sektörde birçok kullanımı olabileceği sonucuna ulaşılmıştır.

Antibacterial Characteristics of Nanofiber Structures Obtained by Benzalkonium Chloride Additive Poly (Vinyl Alcohol)/Gelatin

Polyvinyl alcohol (PVA) is a hydrophilous and semi-crystallized. It has attracted much relevancy due to its pretty chemical endurance, finethermal determination, decent physical specialities, wonderful bio-compatibility and cheapness. Gelatin (G) is a natural polymer and thatare interesting materials for biomedical applications. Electrospinning is a simple method that provides very porous nanofiber productionwith high surface area. It is possible to produce biomedical, filtration, energy storage and protective materials by using electrospinningmethod. Benzalkonium chloride (BAC) is a kind of anti-microbial cationic surface-active agent, which has been pretty used in merchantwound dressings and has a powerful status toward Gr+ bacteria.In this study, nanofibers were produced from electrospinning of BAC, PVA and G containing solutions at various concentrations. The fibersof obtained nanofiber structure were uniform, continuous and intensive. The optimum parameters in terms of good mechanical and antibacterialproperties were determined. S2 sample, electrospun from 11.63% PVA + 1.0% G +1.0% BAC containing solution, had the bestmorphological and mechanical properties due to having the thinnest fiber diameter (51±13nm) and the highest vertical strength (4.299MPa)and horizontal strength (4.058MPa). It also had antibacterial activity against all the bacteria tested (E. coli, P. aeruginosa, B. subtilis andS. aureus). Due to owning good mechanical and antibacterial properties, S2 sample can have many uses in medical sector.

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