Mustafa KÜÇÜKYAZICI, Neşe KEKLİKCİOĞLU ÇAKMAK, Atakan EROĞLU

Synthesis and stability analysis of folic acid-graphene oxide nanoparticles for drug delivery and targeted cancer therapies

Cancer is the growth and proliferation of damage-ending cells in an uncontrolled or abnormal way. Today, it takes place among the most important health problems around the world and in our country. Surgery, radiotherapy, and chemotherapy are the main treatment methods in cancer treatment. The development of resistance to chemotherapeutic medicines has led scientists to investigate this issue as well as the drug’s ability to reach the targeted tumor site and destroying cancer cells in addition to normal cells. The production of various nanostructures for anticancer drug development has been one of the most important areas of nanomedicine. Thus, in the present research, the improved Hummers’ method was employed for the synthesis of graphene oxide nanoparticle (NGO), and it was activated by the folic acid (FA) antibody to increase targeting ability after attachment of the drug to the nanostructure systems. SEM, FTIR, XRD, UV/Vis spectroscopy, and zeta potential analysis were performed for characterization of the products. The highest absorbance of the FA-NGO/DIW nanostructures produced at the concentration of 0.01 mg/ml-0.05 mg/ml synthesized by the Hummers’ method and in the UV/Vis spectra, peaks at 232 nm and 270 nm corresponds to NGO-DIW and FA-NGO/DIW, respectively. The zeta potential value above 35 mV was obtained in all measurements, and the NGO-DIW and NGO-FA-DIW samples maintained stability for days. These findings are consistent with the few studies in the literature, and this study will guide future studies in which nanoparticle systems will be directed to the target by binding chemotherapeutic drugs.

Keywords:

Cancer, nano-graphene oxide folic acid, nano-drug delivery systems, zeta potential,

PDF

___

1. Warheit, D. B., Sayes, C. M., Reed, K. L., & Swain, K. A. Health effects related to nanoparticle exposures: environmental, health and safety considerations for assessing hazards and risks. Pharmacology & therapeutics, 2008, 120 (1), 35-42.
2. Gonzalez, L., Lison, D., & Kirsch-Volders, M. Genotoxicity of engineered nanomaterials: a critical review. Nanotoxicology, 2008, 2(4), 252-273.
3. Geim, Andre K., and Konstantin S. Novoselov. The rise of graphene, Nature materials, 2007, 6.3, 183.
4. Neto, A. C., Guinea, F., Peres, N. M., Novoselov, K. S., & Geim, A. K. The electronic properties of graphene. Reviews of modern physics, 2009, 81(1), 109.
5. Becerril, H. A., Mao, J., Liu, Z., Stoltenberg, R. M., Bao, Z., & Chen, Y. Evaluation of solution-processed reduced graphene oxide films as transparent conductors. ACS nano, 2008, 2(3), 463-470.
6. Stankovich, S., Dikin, D. A., Dommett, G. H., Kohlhaas, K. M., Zimney, E. J., Stach, E. A., ... & Ruoff, R. S. Graphene-based composite materials. nature, 2006, 442(7100), 282.
7. Wang, C., Li, D., Too, C. O., & Wallace, G. G. Electrochemical properties of graphene paper electrodes used in lithium batteries. Chemistry of Materials, 2009, 21(13), 2604-2606.
8. Pasricha, R., Gupta, S., & Srivastava, A. K. A Facile and Novel Synthesis of Ag–Graphene‐Based Nanocomposites. Small, 2009, 5(20), 2253-2259.
9. Shi, Y., Fang, W., Zhang, K., Zhang, W., & Li, L. J. Photoelectrical Response in Single‐Layer Graphene Transistors. Small, 2009, 5(17), 2005-2011.
10. Lv, X., Huang, Y., Liu, Z., Tian, J., Wang, Y., Ma, Y., ... & Chen, Y. Photoconductivity of Bulk‐Film‐Based Graphene Sheets. Small, 2009, 5(14), 1682-1687.
11. Dhand, V., Rhee, K. Y., Kim, H. J., & Jung, D. H. A comprehensive review of graphene nanocomposites: research status and trends. Journal of Nanomaterials, 2013, 158.
12. Marcano, D. C., Kosynkin, D. V., Berlin, J. M., Sinitskii, A., Sun, Z., Slesarev, A., ... & Tour, J. M. Improved synthesis of graphene oxide. ACS nano, 2010, 4(8), 4806-4814.
13. Sun, Z., Yan, Z., Yao, J., Beitler, E., Zhu, Y., & Tour, J. M. Growth of graphene from solid carbon sources. Nature, 2010, 468(7323), 549.
14. Urbas, K., Aleksandrzak, M., Jedrzejczak, M., Jedrzejczak, M., Rakoczy, R., Chen, X., & Mijowska, E. Chemical and magnetic functionalization of graphene oxide as a route to enhance its biocompatibility. Nanoscale research letters, 2014, 9(1), 656.
15. Yang, K., Feng, L., Hong, H., Cai, W., & Liu, Z. Preparation and functionalization of graphene nanocomposites for biomedical applications. Nature protocols, 2013, 8(12), 2392.
16. Zhang, Y., Sun, C., Kohler, N., & Zhang, M. Self-assembled coatings on individual monodisperse magnetite nanoparticles for efficient intracellular uptake. Biomedical microdevices, 2004, 6(1), 33-40.
17. Deb, A., & Vimala, R. Camptothecin loaded graphene oxide nanoparticle functionalized with polyethylene glycol and folic acid for anticancer drug delivery. Journal of Drug Delivery Science and Technology, 2018, 43, 333-342.
18. Huang, Peng, et al. Folic acid-conjugated graphene oxide loaded with photosensitizers for targeting photodynamic therapy. Theranostics, 2011, 1: 240.
19. Low, Philip S.; Henne, Walter A.; Doorneweerd, Derek D. Discovery and development of folic-acid-based receptor targeting for imaging and therapy of cancer and inflammatory diseases. Accounts of chemical research, 2007, 41.1: 120-129.
20. Qin, X. C., Guo, Z. Y., Liu, Z. M., Zhang, W., Wan, M. M., & Yang, B. W. Folic acid-conjugated graphene oxide for cancer targeted chemo-photothermal therapy. Journal of photochemistry and photobiology B: Biology, 2013, 120, 156-162.
21. Hummers Jr, W. S., & Offeman, R. E. Preparation of graphitic oxide. Journal of the american chemical society, 1958, 80(6), 1339-1339.
22. Kovtyukhova, N. I., Ollivier, P. J., Martin, B. R., Mallouk, T. E., Chizhik, S. A., Buzaneva, E. V., & Gorchinskiy, A. D. Layer-by-layer assembly of ultrathin composite films from micron-sized graphite oxide sheets and polycations. Chemistry of materials, 1999, 11(3), 771-778.
23. Zhang, L., Xia, J., Zhao, Q., Liu, L., & Zhang, Z. Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs. Small, 2010, 6(4), 537-544.
24. Keklikcioğlu Çakmak, N, Temel, Ü, Yapıcı, K. Examination Of Rheological Behavior Of Water-Based Graphene Oxide Nanofluids. Cumhuriyet Science Journal, 2017, 38 (4), 176-183.
25. Szabó, T., Berkesi, O., & Dékány, I. DRIFT study of deuterium-exchanged graphite oxide. Carbon, 2005, 43(15), 3186-3189.
26. Pradhan, S. K., Xiao, B., Mishra, S., Killam, A., & Pradhan, A. K. Resistive switching behavior of reduced graphene oxide memory cells for low power nonvolatile device application. Scientific reports, 2016, 6, 26763.
27. Angelopoulou, A., Voulgari, E., Diamanti, E. K., Gournis, D., & Avgoustakis, K. Graphene oxide stabilized by PLA–PEG copolymers for the controlled delivery of paclitaxel. European Journal of Pharmaceutics and Biopharmaceutics, 2015, 93, 18-26.
28. Reed, B. W., & Sarikaya, M. Electronic properties of carbon nanotubes by transmission electron energy-loss spectroscopy. Physical Review B, 2001, 64(19), 195404.

International Advanced Researches and Engineering Journal-Cover

Yayın Aralığı: Yılda 3 Sayı
Başlangıç: 2017
Yayıncı: Dr. Ceyhun YILMAZ

Arşiv