Aziz PAŞAHAN, Canbolat GÜRSES, Melek UYAN, Ali KURUÇAY

Gözyaşı İçeren Farklı Kremlerin Hazırlanması ve Antibakteriyel Özelliklerinin İncelenmesi

Çalışmamızda, birçok enfeksiyondan gözlerimizi koruyan gözyaşının antibakteriyel özelliği Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus haemolyticus ve Staphylococcus epidermidis bakterileri kullanılarak incelenmiştir. Ayrıca, antibakteriyel etkisi tarih boyunca bilinip kullanılan zeytinyağı ve limon suyunun, gözyaşı ile karıştırılmasıyla hazırlanan petrol jelatini olarak da bilinen vazelin içeren farklı formülasyondaki kremlerin antibakteriyel etkisi araştırılmıştır. Kirby-Bauer yöntemi olarak da bilinen inhibisyon zon çapları ölçülerek antibakteriyel etki araştırılmıştır. Sadece gözyaşı uygulanan Staphylococcus epidermidis hariç tüm örneklerin antibakteriyel etki gösterdikleri belirlenmiştir. En yüksek antibakteriyel aktivite, gözyaşı içeren krem (vazelin ve zeytinyağı) ile Pseudomonas aeruginosa ve Staphylococcus epidermidis bakterileri için gözlenmiştir. Ek olarak, Bacillus subtilis ve Staphylococcus haemolyticus bakterilerine karşı en yüksek antibakteriyel etkiyi gözyaşı içeren krem (vazelin, zeytinyağı ve limon suyu) göstermiştir.

Anahtar Kelimeler:

Antibakteriyel aktivite, Gözyaşı, İnhibisyon zon çapı, Vazelin, Zeytinyağı

THE PREPARATION OF VARIOUS TEAR-CONTAINING CREAMS AND INVESTIGATION OF THEIR ANTIBACTERIAL PROPERTIES

In our research, the antibacterial properties of tear which protect our eyes from many infections have been investigated by using Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus haemolyticus and Staphylococcus epidermidis bacteria. In addition, the antibacterial effect of creams in different formulations containing vaseline, also known as petroleum jelly, prepared by mixing olive oil and lemon juice which have been known and used throughout history with tears have been investigated. The antibacterial effect has been investigated measuring the zone of inhibition diameters, also known as the Kirby-Bauer method. It has been determined that all samples showed antibacterial effects except Staphylococcus epidermidis to which only tear was applied. The highest antibacterial activity with tear containing cream (vaseline and olive oil) has been observed for Pseudomonas aeruginosa and Staphylococcus epidermidis bacteria. In addition, tear containing cream (vaseline, olive oil and lemon juice) has shown the highest antibacterial effect against Bacillus subtilis and Staphylococcus haemolyticus bacteria.

Keywords:

Antibacterial activity, Olive oil, Tear, Vaseline, Zone of inhibition,

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