Eryngium Billardieri F. Dolaroche'den (Boğa Dikeni) Hazırlanan Ekstraktlarin Fenolik İçerikleri Ve Biyolojik Özellikleri

Bu çalışmada, Eryngium billardieri (Boğa Dikeni) Ağrı iline bağlı Patnos ilçesinden vejetasyon döneminde toplandı. Toplanan bitkinin bir kısmı -86oC dondurucuda taze olarak depolanırken, diğer bir kısmı ise herbaryum materyali halinde saklandı. Tür tayini yapıldıktan sonra bitkinin taze ve herbaryum numunelerinin toprak üstü kısımlarından metanol ve saf su ekstraktları hazırlandı. Ekstraktların fenolik içeriği HPLC kullanılarak belirlendi. Ayrıca ilgili özütlerin çeşitli in vitro antioksidan özellikleri ve DNA koruyucu özellikleri test edildi. Nihayetinde, elde edilen sonuçlar biribiri ile ilişkilendirilmiştir. Araştırma sonuçları taze bitki ekstraktlarının herbaryum ekstraktlarına göre daha zengin fenolik madde içerdiğini gösterdi. Her iki ekstre iyi antioksidan aktivite gösterse de aktiviteleri standart antioksidanlardan daha düşüktü. Ayrıca herbaryum ekstraktlarının ortamdaki H2O2 varlığından bağımsız olarak, pUC18 DNA stabilizasyonu üzerinde nispeten daha olumlu bir etkiye sahip olduğu görüldü. Literatürde E.billardieri üzerinde yapılan çalışma sayısının sınırlı olduğu göz önüne alınırsa, bu çalışma bu konuda yapılacak çalışmalarda yol gösterici olacaktır.

Anahtar Kelimeler:

Antioksidan, Apiaceae, DNA, Eryngium billardieri, HPLC

BIOLOGICAL PROPERTIES AND PHENOLIC CONTENTS OF EXTRACTS PREPARED FROM ERYNGIUM BILLARDIERI F. DOLAROCHE

In this study, Eryngium billardieri was collected from Patnos district of Ağrı province during the vegetation period. While some of the collected plant was stored fresh in a -86oC freezer, the other part was preserved as herbarium material. After the species of the plant were identified, methanol and pure water extracts were prepared from the aerial parts of the fresh and herbarium samples. The phenolic contents of the extracts were determined by using HPLC. Moreover, various in vitro antioxidant properties and DNA protective properties of the relevant extracts were tested. The comparison of the test results showed that the phenolic compounds of the fresh plant extracts were more than that of herbarium extracts. Although both extracts showed good antioxidant activities, their activity was lower than the standart antioxidants. Moreover, herbarium extracts were seen to have a relatively better effect on the pUC18 DNA stabilization, irrespective of the presence of H2O2 in the environment. Having considered that there is limited number of studies on E.billardieri in the literature, this study will offer important insights into the studies in this area.

Keywords:

Antioxidant, Apiaceae, DNA, Eryngium billardieri, HPLC,

PDF

___

Alan, Y., Savcı, A., Koçpınar, E. F., Kurşat, M., Topdemir, S., Karataş, M., Çakmak, B. (2019). Antimicrobial, antioxidant and DNA protective effects and phenolic content of Lallementia canescens (L.) Fisch. & CA Mey. and Lallementia peltata (L.) Fisch. & CA Mey. Biyolojik Çeşitlilik ve Koruma, 12(3), 78-88.
Ayuso, M., Pinela, J., Dias, M. I., Barros, L., Ivanov, M., Calhelha, R. C., … Gallego, P. P. (2020). Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay. Industrial Crops and Products, 148, 112325.
Benmerache, A., Magid, A. A., Berrehal, D., Kabouche, A., Voutquenne-Nazabadioko, L., Messaili, S., … Kabouche, Z. (2016). Chemical composition, antibacterial, antioxidant and tyrosinase inhibitory activities of glycosides from aerial parts of Eryngium tricuspidatum L. Phytochemistry Letters, 18, 23-28.
Bilal, Ç. (2020). Renal hücrelerde metoreksat kaynaklı sitotoksisite: Kurkumin'in koruyucu rolü. İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 8(2), 281-292.
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199.
Budak, H., Ceylan, H., Kocpinar, E. F., Gonul, N., Erdogan, O. (2014). Expression of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in oxidative stress induced by long-term iron toxicity in rat liver. [Research Support, Non-U.S. Gov't]. J Biochem Mol Toxicol, 28(5), 217-223. doi: 10.1002/jbt.21556
Budak, H., Gonul, N., Ceylan, H., Kocpinar, E. F. (2014). Impact of long term Fe(3)(+) toxicity on expression of glutathione system in rat liver. [Research Support, Non-U.S. Gov't]. Environ Toxicol Pharmacol, 37(1), 365-370. doi: 10.1016/j.etap.2013.12.007
Ebrahimzadeh, M. A., Nabavi, S. F., Nabavi, S. M. (2009). Antioxidant activity of leaves and inflorescence of Eryngium caucasicum Trautv at flowering stage. Pharmacognosy Research, 1(6), 435.
Gulcin, I. (2006). Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). [Comparative Study]. Toxicology, 217(2-3), 213-220. doi: 10.1016/j.tox.2005.09.011
Hançer, Ç. K., Uruşak, E. A. (2017). Apiaceae familyası meyve anatomisindeki “Vitta” terimi ve yerleşimleri. Avrasya Terim Dergisi, 5(2), 19-24.
Kholkhal, W., Ilias, F., Bekhechi, C., Bekkara, F. A. (2012). Eryngium maritimum: A rich medicinal plant of polyphenols and flavonoids compounds with antioxidant, antibacterial and antifungal activities. Current Research Journal of Biological Sciences, 4(4), 437-443.
Kikowska, M., Dworacka, M., Kędziora, I., Thiem, B. (2016). Eryngium creticum-ethnopharmacology, phytochemistry and pharmacological activity. A review. Revista Brasileira de Farmacognosia, 26(3), 392-399.
Kikowska, M., Thiem, B., Szopa, A., Klimek-Szczykutowicz, M., Rewers, M., Sliwinska, E., Ekiert, H. (2019). Comparative analysis of phenolic acids and flavonoids in shoot cultures of Eryngium alpinum L.: an endangered and protected species with medicinal value. Plant Cell, Tissue and Organ Culture (PCTOC), 139(1), 167-175.
Kolaç, T., Gürbüz, P., Yetiş, G. (2017). Doğal ürünlerin fenolik içeriği ve antioksidan özellikleri. İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 5(1), 26-42.
Meot-Duros, L., Le Floch, G., Magné, C. (2008). Radical scavenging, antioxidant and antimicrobial activities of halophytic species. Journal of Ethnopharmacology, 116(2), 258-262.
Nebija, F., Stefkov, G., Karapandzova, M., Stafilov, T., Panovska, T. K., Kulevanova, S. (2009). Chemical characterization and antioxidant activity of Eryngium campestre L., Apiaceae from Kosovo. Македонско фармацевтско друштво, ул. Маршал Тито 13б/8, Скопје, Македонија Macedonian Pharmaceutical Society, Marshal Tito 13b/8, Skopje Macedonia, 23.
Oyaizu, M. (1986). Studies on products of browning reaction: antioxidative activity of products of browning reaction. Jpn. J. Nutr, 44(6), 307-315.
Parham, S., Kharazi, A. Z., Bakhsheshi-Rad, H. R., Nur, H., Ismail, A. F., Sharif, S., … Berto, F. (2020). Antioxidant, Antimicrobial and Antiviral Properties of Herbal Materials. Antioxidants, 9(12), 1309.
Paun, G., Neagu, E., Moroeanu, V., Albu, C., Savin, S., Lucian Radu, G. (2019). Chemical and bioactivity evaluation of Eryngium planum and Cnicus benedictus polyphenolic-rich extracts. BioMed Research International, 2019.
Rjeibi, I., Saad, A. B., Ncib, S., Souid, S. (2017). Phenolic composition and antioxidant properties of Eryngium maritimum (sea holly). J Coast Life Med, 5(5), 212-215.
Savci, A., Kocpinar, E. F., Alan, Y., Kursat, M. (2020). Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal, 27(1), 160-170.
Seal, T. (2016). Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India. Journal of Applied Pharmaceutical Science, 6(2), 157-166.
Siddall, T. L., Ouse, D. G., Benko, Z. L., Garvin, G. M., Jackson, J. L., McQuiston, J. M., … VanHeertum, J. C. (2002). Synthesis and herbicidal activity of phenyl‐substituted benzoylpyrazoles. Pest Management Science: Formerly Pesticide Science, 58(12), 1175-1186.
Soumia, B. (2018). Eryngium campestre L.: Polyphenolic and flavonoid compounds; Applications to health and disease. Polyphenols: Mechanisms of Action in Human Health and Disease, 69-79.
Wu, L.-c., Chang, L.-H., Chen, S.-H., Fan, N.-c., Ho, J.-a. A. (2009). Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. LWT-Food Science and Technology, 42(9), 1513-1519.
Zhang, H., Barcelo, J. M., Lee, B., Kohlhagen, G., Zimonjic, D. B., Popescu, N. C., Pommier, Y. (2001). Human mitochondrial topoisomerase I. Proc Natl Acad Sci U S A, 98(19), 10608-10613. doi: 10.1073/pnas.191321998