Hatay’da yetişen “Helichrysum stoechas” ın Fenolik Madde ve Antioksidan Etkilerinin incelenmesi ve Esansiyel Yağ Bileşiminin Belirlenmesi

Bu çalışma, Hatay bölgesinden toplanan H. stoechas’ın esansiyel yağ bileşimi, antioksidan kapasitesi vetoplam fenolik içeriğini belirlemek amacıyla yapılmıştır. H. stoechas ağırlıkça % 13,55 nem içeriğine sahipken,esansiyel yağ içeriği ağırlıkça % 0,3 oranında olup içerdiği on uçucu bileşik GC ile belirlenmiştir. Ağırlıklı olarakuçucu bileşenler rosifoliol % 4,89, 3-metoksi-y-asaron % 34,69, elemisin % 3,24, miristisin % 23,35, apiol %27,38’dir. Metanolde çözünen madde miktarı % 15,3 olarak bulundu. Toplam fenolik madde konsantrasyonu256,55 ± 3,7mg Gallik asit eşdeğeri/g olarak bulunmuştur. DPPH serbest radikal süpürme aktivitesi IC50 değeri2,76 ± 44x10-2 mg mL-1 olarak bulundu. Metanolik ekstrenin inhibisyon değeri BHA ve BHT ile karşılaştırıldı.Uçucu yağ, halüsinojenik özelliğe sahip elemisin ve miristisin açısından zengindir. Elde edilen sonuçlar, metanolikekstraktının fenolik madde bakımından zengin olduğunu ve sentetik antioksidanlar BHT ve BHA’ya yakın birantioksidan etkinliğe sahip olduğunu gösterdi.

Anahtar Kelimeler:

uçucu yağ, Helichrysum stoechas, antioksidant aktivite, toplam fenolik madde

Determination of Essential Oil Composition and Investigation of Phenolic Substance and Antioxidant Effect of “Helichrysum stoechas” from Hatay

This study was carried out to determine the essential oil composition, antioxidant capacity and totalphenolic substance of Helichrysum stoechas which was gathered from Hatay region. H. stoechas had a moisturecontent of 13.55 weight%, while the essential oil content of 0.3 wt% and ten volatile compounds were determinedand identified by GC. Predominantly volatile components are rosifoliol 4.89%, 3-methoxy-γ-asarone 34.69%,elemicin 3.24%, myristicin 23.35%, apiol 27.38%. Methanol soluble fraction was found to be 15.3%. Total phenolconcentration was found 256.55 ± 3.7 mg gallic acid equivalent/g of extract powder. DPPH free radical-scavengingactivity IC50 was found as 2.76 ± 44x10-2 mg mL-1. The inhibition value of methanolic extract was compared withBHA and BHT. The volatile oil is rich in hallucinogenic elemicin and myristicin. Obtained results showed that themethanolic extract was rich in phenolic content and has a close antioxidant activity to the synthetic antioxidantsBHT and BHA.

Keywords:

Helichrysum stoechas, essential oil, antioxidant activity, total phenolic content,

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