Emre YÖRÜK, Özlem SEFER, Ayşe Server SEZER, Zeynep KONUKCU, Elif Sedef DEVELİ

Eugenol’ün Fusarium culmorum Üzerindeki Etkilerinin İncelenmesi

Fusarium culmorum küçük daneli tahıllarda ekonomik kayıplara yol açan hastalık etmenlerinden birisidir.

Anahtar Kelimeler:

Antifungal, Doğrusal büyüme oranı, FcStua, FcMgv1, qPCR

Investigation of the Effects of Eugenol on Fusarium culmorum

Fusarium culmorum is one of the causal agents lead to economic loses in small grain cereals. Inthis study, alterations in spore production, and linear growth rates in F. culmorum 15 isolate subjected to increasedconcentrations of eugenol (0, 200, 400, 800 µg mL-1) were examined. Additionally, the and expression of FcMgv1and FcStuA genes which are essential in asexual stage and cell wall structure formation were also examined testedin F. culmorum eugenol treated and non-treated fungal cultures. F15 isolate subjected to increased concentrations ofeugenol (0, 200, 400, 800 µg mL-1). Minimum inhibitory concentration was determined as 400 µg mL-1 eugenol.In comparison of control and experiment sets, there were significantly different (p<0.01) the decrease in sporeproduction and linear growth rate (LGR) were significantly different (p<0.01). In real time polymerase chainreaction (qPCR) analysis, β-tubulin was used as endogenous control and the expression of FcMgv1 and FcStuAgenes were determined by using cDNAs converted from total RNAs of control and experiment sets were usedin Eva-Green fluorophore dyebased real time PCR to examine the FcMgv1 and FcStuA expression. Significantdifferences (p<0.05) were also determined in fold changes in gene expression. Normalization results showed thatfold changes in FcMgv1 and FcStuA genes were as +4.35±0.25 and +2.04±0.13, respectively. qPCR results werealso confirmed via reverse transcription PCR (RT-PCR) analysis. The late apoptosis was detected in the cells byusing acridine orange/ethidium bromide staining. Findings obtained from this study showed that eugenol have thepotential antifungal effects and it could be used in struggling with head blight disease caused by F. culmorum via itsin vitro antifungal effects. This study presents the original data on about is first report to evaluatinge the phenotypicand transcriptomic effects of pure eugenol compound on F. culmorum.

Keywords:

Antifungal, FcStua, FcMgv1, Linear growth rate, qPCR,

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