Kadriye Gül YURDAGÜL, Sude ATAY, Ümit BİLGİNER, Taki KARSLI, Eymen DEMİR

Pırlak koyunlarında HSP90AA1 gen bölgesindeki genetik varyasyonlar

Amaç: Bu çalışmanın amacı Pırlak koyun ırkında HSP90AA1 geni varyasyonlarının incelenmesidir. Materyal ve Metot: Antalya ilinde ırkı temsil eden sürülerden rastgele seçilen toplam 100 akraba olmayan hayvan Allel-Spesifik Polimeraz Zincir Reaksiyonu (AS-PZR) tekniğiyle genotiplendirilmiş ve genotipler agaroz jel elektorforeziyle tespit edilmiştir. Bulgular: İki allel (C ve G) ve olası üç genotipin (CC, CG ve GG) tespit edildiği HSP90AA1 bölgesinin polimorfik olduğu belirlenmiştir. C ve G allel frekansı sırasıyla 0.39 ve 0.61 idi, genotip frekansı ise 0.183 (CC) ve 0.413 (GG) aralığında değişmiştir. CG genotip frekansı 0.404 olarak hesaplanmıştır. HSP90AA1 bölgesindeki varyasyonların korunması gelecekte ısı stresine karşı yapılacak olan seleksiyon programlarının planlanması için gerekli olacaktır. Sonuç: HSP90AA1 ve benzeri genler yakın gelecekte iklim değişikliğinden kaynaklana ısı stresinin azaltılması için yeni fırsatlar sunabilmektedir. Ayrıca, Pırlak koyunlarında koruma çalışmalarının şekillendirilmesi için çevresel stresörlerle ilişkili diğer genomik bölgeler temelinde daha fazla çalışmanın yapılması gerekmektedir.

Anahtar Kelimeler:

Pırlak koyunu, AS-PZR, karakterizasyon, ısı stresi, ısı toleransı, HSP90AA1, polimorfizm

Genetic Variations in HSP90AA1 Gene Region in Pırlak Sheep Breed

Objective: This study aimed to reveal variations in HSP90AA1 gene region in Pırlak sheep breed. Material and Methods: A total of 100 animals randomly chosen from representative herds raised in Antalya province were genotyped by Allele-Specific Polymerase Chain Reaction (AS-PCR) technique and genotypes were validated via agarose gel electrophoresis. Results: HSP90AA1 region was found to be polymorphic in which two alleles (C and G) and three possible genotypes (CC, CG and GG) were detected. The frequencies of C and G alleles were 0.39 and 0.61, respectively, whereas genotype frequency ranged from 0.183 (CC) to 0.413 (GG). The frequency of CG genotype was calculated as 0.404. Conservation of the genetic variations at HSP90AA1 region will be required for planning selection programs against heat stress in the future. Conclusion: HSP90AA1 and similar genes may offer new opportunities to reduce heat stress caused by global climate change in the near future. Additionally, more genetic studies for the other genomic regions related to environmental stressors should be conducted in Pırlak breed in order to shape conservation studies.

Keywords:

Pırlak sheep, AS-PCR, characterization, heat stress, heat tolerance, HSP90AA1, polymorphism,

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