Avrupa Levreğinde (Dicentrarchus labrax) Karnitin Palmitoiltransferaz 1A (CPT1A) Geni Tanımlanması ve Karakterizasyonu

Amaç: CPT1A, uzun zincirli yağ asil-CoA'nın mitokondriyal matrise girişine müdahale eder. Bu çalışmada, Akdeniz’de kafes kültürü yapılan Avrupa levreklerinde (Dicentrarchus labrax) CPT1A geninin kısmi dizisinde genetik varyasyonlar araştırılmıştır. Materyal ve Yöntemler: 100 Avrupa levreği bireyinde genomik DNA izole edilmiştir. CPT1A geninin kısmi bir bölgesi termal döngüleyici ile çoğaltıldıktan sonra, PCR ürünleri Sanger yöntemi ile dizilenmiştir. Bulgular: Avrupa levreği CPT1A geninde kısmi dizisinde iki SNP belirlenmiştir. g.2080T>A lokusu için sırasıyla %27.04, %49.92 ve %23.04 frekansları ile TT, TA ve AA genotipleri gözlenmiştir. g.2216A>G lokusunun AA, AG ve GG’nin frekansları sırasıyla %1.2, %19.58 ve %79.21 olarak bulunmuştur. Sonuç: Avrupa levreği yüksek ticari değere sahiptir. Bu bulgular, CPT1A genindeki iki SNP'nin, Avrupa levreğindeki yağ asidi bileşimi ile ilgili genomik seleksiyon programları için kullanılabileceğini düşündürmektedir. Anahtar Kelimeler: Yağ asidi, SNP, levrek, varyasyon

Anahtar Kelimeler:

yağ asidi, SNP, levrek, varyasyon

Identification and Characterization of Carnitine Palmitoyltransferase 1A (CPT1A) Gene in European Sea Bass (Dicentrarchus labrax)

Objective: CPT1A intervenes in the access of the long-chain fatty acyl-CoA to the mitochondrial matrix. In this study, we conducted the genetic polymorphisms of the partial sequence of the CPT1A gene in European sea bass (Dicentrarchus labrax) that were cage cultured in the Aegean Sea. Material and Methods: Genomic DNA was isolated from 100 European sea bass individuals. After amplifying a partial region of the CPT1A gene by thermal cycler, PCR products were sequenced via Sanger method. Results: We detected two SNPs in partial sequence of the CPT1A gene in European sea bass. TT, TA and AA genotypes were observed for CPT1A g.2080T>A locus with frequencies of 27.04, 49.92 and 23.04%, respectively. The frequencies of the g.2216A>G locus as AA, AG and GG were found to be 1.2, 19.58 and 79.21%, respectively. The CPT1A g.2080T>A locus was in HWE, whereas the g.2216A>G locus was not in HWE. Conclusion: European sea bass has a high commercial value. These findings suggest that two SNPs in the CPT1A gene could be used for genomic selection programs related to fatty acid composition in European sea bass. Keywords: Fatty acid, SNP, sea bass, variation

Keywords:

fatty acid, SNP, sea bass, variation,

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