Bahri Devrim ÖZCAN, Harun Reşit KILIÇER

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Yem katkısı selülaz enzimlerini üreten termofilik bacillus suşlarının izolasyonu ve enzimlerin kısmi karakterizasyonu

Bu çalışmada, Düziçi sınırları içerisinde bulunan Haruniye Kaplıcasından toplanan topraknumunelerinden üç adet termofilik Bacillus sp. izolasyonu gerçekleştirilmiştir. Bakteriler sırasıylaBacillus sp. HG1, HG 2 ve HG3 olarak isimlendirilmişlerdir. Selülaz üretimi, üreme periyodununbaşlangıcından itibaren HG1 ve HG2 izolatları için 72. saatte, HG3 izolatı için ise 24. saattemaksimum düzeye çıkmıştır. HG 1 ve HG2 selülazları optimum aktivite ler ini 60°Cde gösterirken,HG3 selülazı 70°Cde göstermiştir. Bununla birlikte HG 1 ve H G 3 selülazları optimumaktivitelerini pH 5.0de gösterirken, HG2 selülazı pH 4.0de göstermiştir. Her üç enzim de60°Cde 30 dakika muhafaza edildiklerinde aktivitelerinin tamamını korurken, daha yükseksıcaklık değerlerinde 30 dk inkübasyon sonucunda aktivite lerini kaybetmeye başlamışlardır. HG 1,HG2 ve HG 3 enzimlerine ait spesifik aktiviteler 55°Cde sırasıyla 3 4. 1 , 67 .8 ve 1 1 2.3 U/mg olarakbelirlenmiştir.

Isolation of feed additive cellulase producing thermophilic bacillus strains and partial characterization of the enzymes

In the present study, we isolated three thermophilic Bacillus strains from the soil samples collectedfrom the Haruniye Thermal Spring located in Düziçi. The isolates were entitled as Bacillus sp.HG1, HG2, and HG 3 , respectively. The maximum cellulase productions were revealed at 72ndhour of incubation period for HG 1 and HG2 strain, and at 24th hour of incubation period for HG3strain, re spectively. The optimum enzyme activity was observed at 7 0°C for HG3 cellulase ,whereas at 60°C for HG1 and HG2 cellulases . On the other hand, optimum pH value for HG2cellulase was 4.0, whereas 5 .0 for HG 1 and HG 3 cellulases . All enzymes protected their activitiesafter pre -incubation at 60°C for 30 min, but they begin to decrease after pre - incubation at highertemperature values. The specific activities of HG 1, HG2, and HG 3 cellulases were 34.1, 67.8 and112.3 U/mg at 55°C, respectively.
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Harran Üniversitesi Ziraat Fakültesi Dergisi-Cover
  • ISSN: 1300-6819
  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: İbrahim TOBİ
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