Nadide NABİL KAMİLOĞLU, Hülya DAĞDELEN HASTÜRK

Streptozotosin ile Diyabet Oluşturulmuş ve Metformin-İnsülin ile Diyabet Tedavisi Gören Ratlarda, Oleuropeinin Hiperglisemi, Total Oksidan ve Total Antioksidan Düzeyleri Üzerine Etkileri

Bu çalışmada, streptozotosin ile diyabet oluşturulmuş ve metformin ve insülin ile diyabet tedavisi gören ratlarda, oleuropeinin hiperglisemi, oksidan-antioksidan düzeyleri üzerine etkilerinin araştırılması amaçlandı. Bu maksatla, 40 adet erkek Sprague Dawley rat kullanıldı. Ratlar her grupta 8 hayvan bulunacak şekilde 4 deney ve 1 kontrol grubu olmak üzere 5 gruba ayrıldı. Kontrol grubuna %0.09 NaCl intraperitoneal (ip) uygulandı. G1’e 50 mg/kg STZ ip, G2’ye 50 mg/kg STZ + 100 mg/kg metformin + 4 İU/kg insülin ip, G3’e 50 mg/kg STZ + 100 mg/kg metformin + 4 İU/kg insülin ip ve 30 mg/kg oleuropein oral olarak ve G4’e 50 mg/kg STZ ip olarak ve 30 mg/kg oleuropein oral olarak uygulandı. Streptozotosin uygulandıktan sonraki 3, 10 ve 21. günlerde deneme gruplarının kan glukoz değerleri kontrol grubuna göre istatistiksel olarak önemli düzeyde yüksek bulunmuştur (sırasıyla P<0.001, P<0.01, P<0.01). Plazma total antioksidan kapasiteleri gruplar arasında kıyaslandığında, G2 ve G3 grubunun Total Antioksidan Kapasite (TAC) düzeyleri, G1 grubuna göre istatistiksel olarak daha yüksek belirlenmiştir (P<0.001). Kontrol grubu ile deneme gurupları arasında kıyaslama yapıldığında G3 grubunun TAC düzeylerinin, kontrol grubuna göre istatistiksel olarak yüksek olduğu belirlenmiştir (P<0.05). G1 (P<0.001), G2 (P<0.01) ve G3 (P<0.05) grubunun plazma Total Oksidan Kapasitelerinin (TOC) kontrol grubuna göre istatistiksel olarak yüksek olduğu belirlenmiştir. Deneme gruplarının karaciğer TAC düzeylerinin kontrol grubuna göre istatistiksel olarak önemsiz olduğu tespit edildi. Karaciğer total oksidan kapasiteleri gruplar arasında kıyaslandığında G2 grubu ile G4 gurubunun TOC düzeylerinin kontrol grubuna göre istatistiksel olarak yüksek olduğu (sırasıyla P<0.01, P<0.001) belirlenmiştir. Sonuç olarak oleuropeinin tek başına kullanımının kan glukoz düzeyini, canlı ağırlığı ve plazma ile karaciğer TAC ve TOC kapasitelerini etkilemediği, ancak diyabette yükselen oksidasyona karşılık, metformin ve insülinle birlikte kullanımının antioksidan sisteme destek olduğu ve serbest radikal tutucu etkiyi kuvvetlendirdiği düşünülmektedir.

Anahtar Kelimeler:

Diabet, İnsulin, Metformine, Oleuropein, TAC, TOC

Effects of Oleuropein on Hyperglycemia, Total Oxidant and Antioxidant Capacity in Streptozotosin-Diabetic Rats Treated with Metformin and Insulin

In this study, it was aimed to investigate the effects of oleuropein on hyperglycemia and oxidant-antioxidant levels in metformin-insulin treated streptozotocin-diabetic rats. For this purpose, 40 Sprague-Dawley rats were used. Animals were divided into 5 groups as 4 experimental and 1 Control each containing 8 rats. 1 ml isotonic NaCl solution was injected intraperitoneally (ip) to the Control group while 50 mg/kg STZ ip was given to the G1, 50 mg/kg STZ + 100 mg/kg Metformin + 4 IU/kg insulin ip was given to the G2, 50 mg/kg STZ + 100 mg/kg Metformin + 4 IU/kg insulin ip and 30 mg/kg orally oleuropein was given to the G3 and 50 mg/kg STZ ip and 30 mg/kg oleuropein orally was given to the G4. Blood glucose values were statistically higher in the experimental groups than the control after streptozotocin administration (P<0.001, P<0.01, P<0.01 respectively). Plasma Total Antioxidant Capacity (TAC) of G2 and G3 were statistically higher than G1 (P<0.001). It was found that plasma TAC levels of G3 were statistically higher than the control (P<0.05). It was determined that the G1 (P<0.001), G2 (P<0.01) and G3 (P<0.05) group had significantly higher plasma Total Oxidant Capacity (TOC) than the control group. TAC levels in the liver of the experimental groups were determined to be statistically insignificant according to the control group. The liver TOC levels of the G2 and G4 group were significantly higher than the control group (P<0.01, P<0.001 respectively). In conclusion, the use of oleuropein alone did not affect the blood glucose level, the live weight, the plasma and the liver TAC and TOC levels, but the use of metformin and insulin with oleuropein as an antioxidant and free radical scavenger following to the rising oxidation in diabetes were supported and strengthen the system.

Keywords:

Diabet, Insulin, Metformine, Oleuropein, TAC, TOC,

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