Koray TEKİN, Çiğdem ÇEBİ ŞEN, Ergun AKÇAY

Effect of Egg Yolk and Removal of Seminal Fluid on Semen Cryopreservation in Norduz Goat

Bu çalışma, santrifüj edilen ve santrifüj edilmeyen norduz teke spermasının farklı oranlarda yumurta sarısı içeren yağsız süt tozu sulandırıcısı ile sulandırılıp dondurulması ve dondurma sonrası spermatolojik özelliklerin saptanması amacıyla yapılmıştır. Çalışmada, 5 baş ergin Norduz tekesinden alınan ejakulatlar kullanıldı. Teklerden, çiftleştirme mevsiminde suni vagina kullanılarak haftada iki kez olmak üzere toplam 20 ejakulat toplandı. Alınan ejakulatlardan her biri 4 eşit hacme bölündü. Tüplerden ikisi seminal plazmanın uzaklaştırılmasını sağlayan santrifüj işlemi için yıkama solüsyonu ile sulandırılırken diğer 2 tüp santrifüj edilmedi. Yıkama işlemi için her bir ejakulat 1:9 oranında yıkama solüsyonuyla sulandırıldı ve oda ısında 600 g'de 10 dakika santrifüj edilerek süpernatant uzaklaştırıldı. Santrifüj edilen ve santrifüj edilmeyen sperma örnekleri sırasıyla % 5 ve % 10 oranında yumurta sarısı içeren yağsız süt tozu sulandırıcısıyla sulandırılarak 0.25'lik payetlere çekildi. Dondurma işlemi sıvı azot buharında gerçekleştirildi ve sıvı azot içinde saklandı. Çözdürme sonrası motilite ve total anormal spermatozoa oranı değerlendirildi. Seminal plazmayı ayırma işleminin motilite ve total anormal spermatozoa oranı üzerine zararlı etkisi olduğu gözlendi. Diğer yönden, % 10 yumurta sarısı motilite ve total anormal spermatozoa oranını olumlu yönden etkiledi (p

NorduzTekesiSperma sınınD ondurulmasında Seminal Plazmanın Uzaklaştırılmasının ve YumurtaSarısının İlavesinin Etkisi

Cryopreservation of goat spermatozoa is a valuable tool for genetic management. Previous studies have shown the frezeeablity of spermatozoa from various goat breeds but also have difficulties with available protocols. Therefore, we want to investigate the effects of centrifugation and different egg yolk ratios (5% and 10%) on spermatological parameters of Norduz goat spermatozoa. A total number of 20 ejaculates were collected with an artificial vagina from 5 mature Norduz male goats (3 and 4 years of age) twice a week, during the breeding season twice a week. Each ejaculate were divided into four equal aliquots. The samples were then diluted 1:9 (v:v) in washing solution, centrifuged at 600 g for 10 min at room temperature and the supernatant discarded for sperm washing process. Fresh and washed samples then were diluted with skim milk containing % 5 egg yolk and %10 egg yolk, respectively. Extended samples were loaded into 0.25 ml straws, equilibrated at that 4 C for 2h, frozen in nitrogen vapour for 10 min and plunged into liquid nitrogen. Samples were assessed for motility and acrosome integrity upon collection and after freezing and thawing. Removel of seminal fluid had detrimental effects on motility and percentage of total abnormal spermatozoa (p

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