Stage II Kolon kanseri dokularında Onkogenik microRNA olan miR-92, miR- 21, miR-155'in qRT-PCR'da Ekspresyonu
Amaç: Bu çalışmada Stage II kolorektal kanserli hastalardan alınmış tümörlü ve normal kolon/rektum dokularında Onkogenik mikroRNA (miRNA) olan miR-92, seviyelerinin qRT-PCR ile tespit edilmesi ve elde edilen verilerin karşılaştırılması amaçlanmıştır. Materyal ve Metod: Bu çalışma Gaziantep Üniversitesi Tıp Fakültesi Araştırma ve Uygulama Hastanesi laboratuar değerlendirme sonucunda kolorektal kanser tanısı konmuş 9 hastanın tümörlü ve normal (kontrol-temiz cerrahi sınırları) kolon/rektum dokular ile çalışılmıştır. qRT-PCR uygulaması için miRNeasy mini kit ile izole edilen miRNA'lar kullanıldı. İzole edilen miRNA'lar miScript RT-PCR ticari kit ile cDNA'ya dönüştürüldü. Doku örneklerindeki miRNA ekspresyon seviyeleri ile cDNA örnekleri RT- SYBR Green qPCR kiti kullanılarak Rotor Gene RT-PCR sistemi ile belirlendi. Bulgular: Kolorektal kanser teşhisi konulmuş 9 hastanın ortalama miRNA-21 ekspresyon seviyesi normal doku ve tümörlü doku karşılaştırıldığında 2.09 kat arttığı, miR-155'in ise 2.22 kat arttığı tespit edilmiştir. Bu artış istatiksel olarak anlamlı bulunmuştur (p
Expression of miR-92, miR-21, miR-155, Oncogemic miRNAs using qRT-PCR in Colon Cancer stage II Tissues
Background: In this study, we aimed to detect the expression levels of miR-92, miR-21 and miR-155 in tumor and normal colon and rectum tissues with oncogenic microRNA (miRNA) obtained from patients with Stage II colorectal cancer using qRTPCR and to compare the obtained data. Material and Method: This study was carried out with the clinical, laboratory and pathological findings of the tissues obtained from patients diagnosed with colorectal cancer at Gaziantep University Medical Faculty Research and Training Hospital -General Surgery Department. miRNeasy mini kit-isolated miRNAs were used for qRT-PCR implementation. The isolated miRNAs were converted into cDNA via the miScript RT-PCR commercial kit. The cDNA samples with miRNA expression levels in tissue samples were identified via Rotor Gene RT-PCR system using RT-SYBR Green qPCR kit. Result: The mean level of miRNA-21 expression in 9 patients diagnosed with colorectal cancer appears to have increased 2.09 fold compared to normal tissue and tumor tissue, while miR-155 increased 2.22 fold. This increase was considered as statistically significant (p 0.05).Conclusion: According to the findings obtained in ourstudy, the expression levels of oncogenic miRNA miR21and miR-155 in tumor colon tissues of patients withcolorectal cancer have increased. The results of recentstudies have shown that miRNAs involved in theregulation of many cellular functions may be used asbiomarkers for diagnosis and treatment of cancer.
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- ISSN: 1304-9623
- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 2004
- Yayıncı: Harran Üniversitesi Tıp Fakültesi Dekanlığı
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