Şanlıurfa’daki Prostat Kanseri Hastalarında VGSC’lerin İmmünohistokimyasal Olarak İncelenmesi
Amaç: Bu çalışmada, genitoüriner sistem
rahatsızlıkları nedeniyle Şanlıurfa'daki Harran
Üniversitesi Tıp Fakültesi Hastanesi ve Mehmet Akif
İnan Eğitim ve Araştırma Hastanesi’ne başvuran bazı
hastalardan alınmış prostat doku örneklerinde Nav1.7
geni tarafından kodlanan proteinin varlığının
araştırılması amaçlanmıştır.
Materyal ve Metod: Çalışmada, tanı konulmuş
(benign prostatik hiperplazi, prostatik intraepitelyal
neoplazi, metastatik olmayan prostat kanseri ve
metastatik prostat kanseri) 20 hastaya ait prostat
dokusu örnekleri ve dört normal prostat kullanıldı.
Harran Üniversitesi Tıp Fakültesi Hastanesi ile
Mehmet Akif İnan Eğitim ve Araştırma Hastanesi'nin
Patoloji arşivlerinden alınan bu numuneler 2012–2014
yıllarına aittir. İşaretleme için anti-sodyum kanalı
Nav1.7 monoklonal antikor (Millipore, Merck, ABD)
kullanıldı. Pozitif kontrol olarak, üretici firmanın
önerisine göre, sıçan beyincik dokusu ile çalışıldı.
İmmunohistokimyasal boyama işlemi otomatik
immünhistokimya cihazında (Ventana, Roche, ABD)
yapıldı.
Bulgular: Primer antikor uygulanmayan negatif
kontrol kesitlerinde boyanma olmadı. Pozitif kontrol
ile karşılaştırıldığında prostat doku örneklerinde
(normal prostat dokusu, benign prostatik hiperplazi,
prostatik intraepitelyal neoplazi dokusu, metastatik
olmayan prostat kanseri ve metastatik prostat kanseri)
dikkate değer bir boyanma gerçekleşmediği
gözlemlendi.
Sonuç: Nav1.7 geni tarafından kodlanan VGSC
proteini, normal prostat ve benign prostat hiperplazisi
yanı sıra prostatik intraepitelyal neoplazi, metastatik
olmayan prostat kanseri ve metastatik prostat kanseri
gibi farklı derecelerde kanser bulunan hastalarda
immünohistokimyasal olarak gösterilememiştir.
İncelemeler, diğer genler tarafından kodlanan
VGSC'lere karşı üretilmiş çeşitli antikorlarla
işaretlenerek sürdürülmelidir. Ayrıca bulgular diğer
moleküler tekniklerle de doğrulanmalıdır.
An Immunohistochemical Investigation of VGSCs in Prostate Cancer Patients in Sanliurfa
Aim: In this study, it was aimed to investigate presence
of the protein which encoded by Nav1.7 gene in
prostate tissue specimens obtained from some patients
consulted to Harran University Medical Faculty
Hospital and Mehmet Akif Inan Training and Research
Hospital in Sanliurfa owing to genitourinary system
disorders.
Materials and Methods: In this study, it was used the
prostate tissue samples which belong to 20 diagnosed
patients (benign prostatic hyperplasia, prostatic
intraepithelial neoplasia, non-metastatic prostate
cancer and metastatic prostate cancer), and four normal
prostate. These specimens obtained from Pathology
Department archives of Harran University Faculty of
Medicine Hospital and Mehmet Akif Inan Training and
Research Hospital belong to 2012–2014. For labelling,
Anti-Sodium channel Nav1.7 monoclonal antibody
(Millipore, Merck, USA) was used. It was studied with
rat cerebellum tissue as positive control in accordance
with the manufacturer’s protocol. The
immunohistochemical staining procedure was
performed in automatic immunohistochemistry device
(Ventana, Roche, USA).
Results: There was no staining in the negative control
sections which non-treated with primer antibody. No
remarkable staining was observed in prostate tissue
specimens (normal prostate tissue, benign prostatic
hyperplasia, prostatic intraepithelial neoplasia tissue,
non-metastatic prostate cancer and metastatic prostate
cancer) when compared with the positive control.
Conclusion: The VGSC protein which encoded by
Nav1.7 gene was not showed by
immunohistochemically in patients with normal
prostate, benign prostatic hyperplasia as well as
different grades of cancer such as prostatic
intraepithelial neoplasia, non-metastatic prostate
cancer, and metastatic prostate cancer. Investigations
should be continued by marking with various
antibodies that produced against VGSCs encoded by
other genes. Additionally, the findings should confirm
with other molecular techniques.
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