Faruk SÜZERGÖZ, Ali O GÜROL, Fatih M EVCİMİK, Nevin YALMAN

Fankoni anemili hastaların mitojenlere, bakteriyel ve viral antijenlere karşı in vitro lenfoproliferatif yanıtları

Fankoni anemili hastaların enfeksiyonlara karşı artmış hassasiyeti, hastaların mitojenlere, bakteriyel ve viral antijenlere karşı immün cevap yetenekleri in vitro ortamda belirlenmek suretiyle incelenmiştir. Periferik kan mononükleer hücreleri PBMC 11 Fankoni anemili hasta ve 11 sağlıklı kontrol bireyinden alınmıştır. Her bir örnekten 105 hücre alınarak MTT yöntemiyle, 20 µg/ml fitohemaglutinin, PHA , 10 µg/ml pokeweed mitojen PWM , 6 µg/ml Tetanoz toksoidi TT , 10 µg/ml saflaştırılmış mycobacterium proteini PPD and 1.25 µg/ml citomegalovirus CMV antijeni ile test edilmiştir. PHA, PWM, TT ve PPD’ ye karşı lenfoproliferatif yanıt kontrol grubuna göre Fankoni anemili hastalarda anlamlı şekilde düşük bulunmuştur. CMV seropozitif hastalarda ise CMV antijenine karşı lenfoproliferatif yanıt CMV seronegatif hastalardan daha yüksek bulunmuştur. Sonuç olarak, Fankoni anemili hastalarda mitojenlere, bakteriyel ve viral antijenlere karşı düşük lenfoproliferatif yanıtın infeksiyonlara yatkınlığın bir işareti olduğunu fakat CMV seropozitif hastalarda görüldüğü gibi antijenle temas durumunda hücrelerin immunopotent hale dönüşebildiğini söyleyebiliriz

Anahtar Kelimeler:

Fanconi anemisi, lenfoproliferasyon, mitojen, citomegalovirus

Lymphoproliferative response of Fanconi anemia patients to mitogens, bacterial and viral antigens in vitro

Increased susceptibility to infections in Fanconi anemia FA have been investigated by determining immune response capability of the patients against to mitogenic, bacterial and viral antigens in vitro. Peripheral blood mononuclear cells PBMC were obtained from 11 FA patients and 11 healthy control individuals. 105 cells from each sample were assessed by using colorimetric MTT assay including 20 µg/ml pytoheamagglutinine PHA , 10 µg/ml pokeweed mitogen PWM , 6 µg/ml Tetanus toxoid TT , 10 µg/ml purified protein derivative of mycobacterium PPD and 1.25 µg/ml cytomegalovirus CMV antigen. Lymphoproliferative response to PHA, PWM, TT and PPD were found significantly lower in FA patients than control group. Lymphoproliferation to CMV antigen was significantly higher in CMV seropositive than seronegative FA patients. To conclude, the lower lymphoproliferative response capability to mitogens and bacterial antigens in FA patients implied susceptibility to infections, but, it is likely in CMV seropositive FA patients, the cells encountering antigens could become immunopotent

Keywords:

Fanconi anemia, lymphoproliferation, mitogen, cytomegalovirus,

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