Voltametrik Epinefrin Tayinine Yönelik Hambeles (Myrtus communis L.) Doku Homojenatı Temelli Biyosensör Sistemi Geliştirilmesi

Farmasotik numunelerde epinefrinin (EP) voltametrik tayini için bitki dokusuna dayalı yeni bir biyosensor geliştirildi. Doku homojenatı, camsı karbon üzerinde glutaraldehit ile çapraz bağlanarak immobilize edildi. Yaban mersininde (Myrtus communis L.) bulunan polifenol oksidaz enzimleri, epinefrinin epinefrinkinon’a oksidasyonunu katalize eder. Optimize edilmiş çeşitli operasyonel parametreler altında, biyosensor 10- 100 μM aralığında doğrusal bir yanıt gösterdi. Tespit limiti (LOD), 3.2x10-6 mol L-1 (3.2 uM) (eğim başına 3σ) olarak hesaplandı. 5.0 x 10-5 mol L-1 EP’nin yedi ardışık ölçüm sonucu için göreli standart sapma (RSD) % 4.6 olarak hesaplandı. Biyosensor, 4°C’de bir fosfat tamponunda 11 gün depolandıktan sonra %70 etkinliğini muhafaza ettiği için çok iyi stabiliteye sahiptir. Bu biyosensorun uygulanabilirliği, gerçek numunelerin analizi ile gösterildi ve biyosensor tarafından elde edilen sonuçlar ile spektrofotometrik yöntemle ölçülen sonuçlar arasında iyi bir korelasyon elde edildi. Myrtle doku homojenatına dayalı biyosensor ile elde edilen bu olumlu sonuçlar, hazırlanmasının basitliği ve düşük maliyetiyle birleşti ve bu prosedürleri farmasotik ürünlerdeki EP kantifikasyonu için cazip hale getirildi.

Development of a Biosensor Based on Myrtle (Myrtus communis L.) Tissue Homogenate for Voltammetric Determination of Epinephrine

Aplant tissue based biosensor was proposed for voltammetric determination of epinephrine (EP) in pharmaceutical samples. The tissue homogenate was immobilized by crosslinking with glutaraldehyde on the glassy carbon. The polyphenol oxidase enzymes present in fibers of a myrtle tree fruits maintained high bioactivity on this biomaterial, catalyzing the oxidation of epinephrine to epinephrinequinone. Under optimize working conditions, the biosensor showed a linear response in the range of 10–100 µM. The limit of detection (LOD) was calculated as 3.2 × 10−6 mol L-1 (3.2 µM) (3σper slope). The reproducibility, expressed as the relative standard deviation (RSD) for seven consecutive determinations of 5.0 × 10-5 mol L-1 EP was 4.6%. The biosensor retained 70% activity after 11 days of storage in a phosphate buffer at 4°C. The applicability of this biosensor was demonstrated with the analysis of real samples and a good correlation was obtained between results acquired by the biosensor and those measured by spectrophotometric method. Such favorable results obtained with the myrtle tissue homogenate based biosensor, joined with the simplicity and low-cost of its preparation turns these procedures very attractive for EP quantification in pharmaceutical products.

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