Poli(α-Metil β-Propiolakton)’un Sentezi, Karakterizasyonu ve Biyobozunurluğu

Poli(α-metil β-propiyolakton) (PmPL) karşılık gelen laktonun anyonik halka açılması polimerizasyonu ile sentezlendi. PmPL’un yapısal karakterizasyonu FTIR, 1 H-NMR ve MALDI-MS spektrometri ile gerçekleştirildi. Polimerin termal davranışı DSC ve TGA ile incelendi. Camsı geçiş, erime ve termal bozunma sıcaklıkları sırasıyla -36.7, 95.5 ve 242.4 oC olarak belirlendi. Biyobozunma çalışmaları 37 oC de pH 7.4 fosfat tamponu (PBS), pancreatic tripsin içeren PBS ve pankreatik lipaz içeren PBS gibi üç farklı ortamda yürütüldü. Polimerin bozunma kinetiği ve mekanizması gravimetrik ölçümler ve LC-MS analizi ile takip edildi. Lipaz ve tripsin içeren tampon ortamlarında, beş haftalık peryotta % 65’e varan kütle kayıpları kaydedildi. Ortamlardan alınan kalıntıların LCMS analizi ile biyobozunmanın büyük oranda ester bağının hidrolizi üzerinden yürüdüğü belirlendi.

Synthesis, Characterization and Biodegradation of Poly(α- Methyl β-Propiolactone)

Poly(α-methyl β-propiolactone) (PmPL) was synthesized through anionic ring-opening polymerization of the corresponding lactone. Structural characterization of PmPL was performed by using FTIR, 1 H-NMR and MALDI-MS spectrometry. Thermal behavior of the polymer was investigated by using DSC and TGA. Glass transition, melting and thermal degradation temperatures of PmPL were determined as -36.7, 95.5 and 242.4 oC, respectively. Biodegradation studies were conducted at 37 oC in three different media, that is, pH 7.4 phosphate buffered saline (PBS), PBS containing pancreatic trypsin and PBS containing pancreatic lipase. The biodegradation kinetics and mechanism of the polymer were followed by gravimetric measurements and LC-MS analysis. Up to 65% mass loss in 5-week period was recorded for the buffer media in the presence of lipase and trypsin. LC-MS analysis of the residues in the media revealed that biodegradation undergoes mainly through ester bond hydrolysis.

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Hacettepe Journal of Biology and Chemistry-Cover

ISSN: 2687-475X
Yayın Aralığı: Yılda 4 Sayı
Başlangıç: 1972
Yayıncı: Hacettepe Üniversitesi, Fen Fakültesi

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