Mehtap SAHİNER, Sultan BUTUN, Duygu ALPASLAN, B. Oral BİTLİSLİ

Potansiyel yara örtü uygulamaları için kollajen esaslı kompozit malzemelerin sentetik polimeler ile hazıranması

K ollajen yaşayan organizmada fibril proteinlerin önemli bir kısmıdır. Hayvan dokularında özellikle tendonların ana yapısı kollajen içerir. Bu çalışmada doğal kollajen coll 2-hidroksil etilmetakrilat HEMA ve akrilamid AAm gibi değişik sentetik monomerler ile modifiye edilerek p Coll-ko-HEMA ve p Coll-ko-AAm şekilde kollajenin doğal karakteristiklerini artırmak için kompozit olarak hazırlanmışlardır. Hazırlanan iç içe geçmiş ağ yapıların IPN biyouyumluluk testleri MTT assay ile yapılmış ve biyouyumlu bulunmuştur. Trimetoprim TMP and Naproksen NP gibi antibakterial aktif ajanlar model ilaç olarak kullanılmış ve sentezlenen kompozithidrojel-kollajen IPN matrikslerinden salım çalışmaları yapılmıştır. İlaç yüklenen IPN-filmleri salım çalışmaları biyomimetik ortamda yapılmıştır. Kollejen-sentetik polimer matrikslerinin kolayca hazırlanabildiği ve aktif ajan salımı için ilaç salım sistemi olarak kullanılabilirliği sonucu çıkarılarak yara örtü uygulamalarınnda büyük potansiyele sahip olduğu bulunmuştur

Anahtar Kelimeler:

Kollajen, kolajen-hidrojel IPN kompozit, ilaç salım cihazları, biyouyumluluk

Preparation of collagen based composite materials with synthetic polymers for potential wound dressing applications

Collagen is very important part of the fibrous proteins in the living organism. Animal tissues, especially tendons main structure contain collagen. In this investigation, natural collagen coll modified with differ- ent synthetic monomers such as 2-hydroxy ethylmethacrylate HEMA and acrylamide AAm was prepared as p Coll-co-HEMA and p Coll-co-AAm composites to enhance collagen natural characteristics. Biocompatibility of the prepared interpenetrating polymeric network IPN was tested with MTT Assay and found biocompat- ible. Model drugs, such as Trimetoprim TMP and Naproxene NP were used as antibacterial active agents for release studies from the synthesized composite hydrogel-collagen IPN matrices. The drug-loaded IPN films release studies were carried out in bio-mimetic media. It was found that collagen-synthetic polymer matrices can be readily prepared and used for drug delivery system in the release of active agents, providing great po- tential in wound dressing applications.

Keywords:

Collagen, collagen-hydrogel IPN composites, drug delivery devices, biocompatibility,

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