Poli hidroksietil metakrilat-N-metakrilo-L-Histidin metil ester Temelli Kriyogel ile Beta talasemili İnsan Plazmasından Fe+3’ün Uzaklaştırması

B u çalışmada, Fe3+ adsorpsiyonu, süpermakrogözenekli poli 2-hidroksietil metakrilat-N-metakrilo- I -histidin metil ester [poli HEMA-MAH ] kriyojeli kullanılarak incelenmiştir. Poli HEMA-MAH kriyojeli elektron mikroskop taraması ve şişme testleriyle karakterize edilmiştir. Akış hızı, pH, Fe3+’ün başlangıç derişiminin Fe3 adsorpsiyonuna etkileri incelenmiştir. Poli HEMA-MAH temelli kriyojelin denge şişme derecesi 7.54 g H0/g kriyojel olarak bulunmuştur. Poli HEMA-MAH temelli kriyojelin Fe3+ adsorpsiyon kapasitesi 1.71 mg/g bulunmuştur. Önemli miktarda adsorpsiyon kapasitesi kaybı olmadan Fe3+ iyonlarının P HEMA-MAH kriyojel ile adsorbe ve desorbe edildiği saptanmıştır

Anahtar Kelimeler:

Fe3+ uzaklaştırılması, Beta Talasemi, Afinite Kromatografisi, Kriyojel, p HEMA-MAH

Poly hydroxyethyl methacrylate -co-N-methacryloyl- L - histidine methyl ester Based Cryogel for the Removal of Fe3+ from Human Plasma effected with Beta Thalassemia

In this study, Fe3+ adsorption was carried by using supermacroporous poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel. Poly HEMA-MAH cryogel was characterized by swelling tests and scanning electron microscopy. The influence of flow rate, pH, initial Fe3+ concentration, and Fe3+ adsorption from human plasma effected with beta thalassemia on the adsorption efficiency of the cryogel was investigated. The equilibrium swelling degree of poly HEMA-MAH cryogel was found to be 7.54 g H2O/g cryogel. Fe3+ adsorption capacity of poly HEMA-MAH cryogel from aqueous solution was estimated as 1.79 mg/g cryogel, while lower adsorption capacity was observed in human plasma 1.71 mg/g . It was also observed that Fe3+ could be repeatedly adsorbed and desorbed using poly HEMA-MAH cryogel without signi- ficant loss in its adsorption capacity.

Keywords:

Fe3+ removal, Beta Thalassemia, Affinity chromatography, cryogel, poly HEMA-MAH,

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