Hülya KARAÇAM, Ekrem TUNCA, Yasemin KAYGISIZ, Gözde İMDAT, Metin BÜLBÜL

Bazı Yenebilir Mantar Ekstrelerinin İnsan Karbonik Anhidraz İzozimleri Üzerine Etkilerinin İncelenmesi

B u çalışmada, dört yenebilir mantar türünün Lentinula edodes, Lactarius deliciosus, Lactarius deterrimus, Terfezia boudieri insan karbonik anhidraz izoenzimleri hCA I ve hCA II üzerine etkileri in vitro olarak incelenmiştir. hCA I ve II izoenzimleri Sepharose®4B-L-tirozin-p-aminobenzen sülfonamit afinite kromatografisi kullanılarak eritrositlerden saflaştırıldı. Daha sonra mantar özütlerinin hCA I ve II izoenzimlerinin hidrataz ve esteraz aktivitelerinin üzerine etkileri ölçüldü. L. edodes hCA I ve II nin esteraz aktiviteleri üzerinde inhibitor etki göstermiştir. Ancak diğer özütler hCA I ve II nin esteraz aktiviteleri üzerinde aktivatör etki göstermişlerdir

Anahtar Kelimeler:

Karbonik anhidraz, hidrataz aktivitesi, esteraz aktivitesi, mantar özütleri

Investigation of The Effects of Some Edible Mushroom Extracts on Human Carbonic Anhydrase Isozymes

In this study the effects of four edible mushroom species Lentinula edodes, Lactarius deliciosus, Lactarius deterrimus, Terfezia boudieri on human carbonic anhydrase isozymes hCA I and hCA II were investiga- ted as in vitro. hCA I and II isozymes were purified from erythrocytes by using Sepharose®4B-L-tyrosine-p- aminobenzene sulfonamide affinity chromatography. Later the effects of the mushroom extracts on the hydra- tase and esterase activities of hCA I and II were measured. L. edodes showed inhibitory effect on the esterase activities of hCA I and II. However, other extracts showed activator effects on the esterase activities of hCA I and II.

Keywords:

Carbonic anhydrase, hydratase activity, esterase activity, mushroom extracts,

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