İrbesartan ve Hidroklorotiyazidin CE-DAD ile Tabletlerden Eşzamanlı Miktar Tayin

İ rbesartan ve hidroklorotiazidin tabletlerde eş zamanlı analizi için bir elektroforetik yöntem geliştirilmiştir. %10 metanol içeren 10 mM borat pH 9.0 çalışma tamponu olarak kullanılmıştır. Bileşikler 6 dakikadan daha düşük sürede ayrılmıştır. Tayin sınırı LOQ değerleri irbesartan için 3.370×10-7 M 0.144 µg/mL ve hidroklorotiazid için 3.140×10-7 M 0.094 µg/mL ’dir. Yöntem, irbesartan ve irbesartan/hidroklorotiazidi birlikte içeren tabletlere yaklaşık %100 gibi bir geri kazanımla başarılı bir şekilde uygulanmıştır

Anahtar Kelimeler:

İrbesartan, hidroklorotiazid, kapiler elektroforez, validasyon

Simultaneous Determination of Irbesartan and Hydrochlorothiazide in Tablets by CE-DAD

Acapillary electrophoretic method was developed and validated for the simultaneous determination of irbesartan and hydrochlorothiazide in tablets. The run buffer was used 10 mM borate buffer containing 10% methanol pH 9.0 . Compounds were separated less than 6 minutes. The limit of quantification LOQ values were 3.370×10-7 M 0.144 μg/mL and 3.140×10-7 M 0.094 μg/mL for irbesartan and hydrochlorothiazide, respectively. The method was applied successfully to both irbesartan and irbesartan/hydrochlorothiazide combi- ned tablets with good recoveries almost 100%.

Keywords:

Irbesartan, hydrochlorothiazide, Capillary electrophoresis, Validation,

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