Katı Faz Fermantasyonu ile Bacillus circulans ATCC 4516’ dan α-Amilazın Üretimi ve Optimizasyonu

B u çalışmanın amacı Bacillus circulans ATCC 4516 tarafından üretilen α-amilazın belirli üretim parametrelerini incelemektir. α-Amilazın optimizasyon parametreleri katı faz fermantasyonu ile gerçekleştirilmiştir. Katı atık besin kaynağı olarak pirinç kepeği kullanılmıştır. Karbon ve metal tuz kaynakları eklenmesi ile enzim üretimi azalmıştır. İnkübasyon zamanı, inkübasyon sıcaklığı, inokülüm düzeyi, başlangıç pH ve ekstraksiyon ortamı gibi belirli fermantasyon parametreleri ayrı ayrı incelenmiştir. Maksimum α-amilaz üretim miktarı 2716.9 ± 35.9 U/mg amonyum klorür eklenmesi ile 48. saatte 37ºC’de inokülüm düzeyi %25, başlangıç pH’ı 7.5 olarak elde edilmiştir

Anahtar Kelimeler:

Bacillus circulans ATCC 4516, pirinç kepeği, α-amilaz, katı faz fermantasyon

Production and Optimization of α-Amylase from Bacillus circulans ATCC 4516 with Solid State Fermentation

The aim of this paper is to study influence of the certain production parameters of α-amylase by Bacillus circulans ATCC 4516. Optimization parameters of α-amylase were carried out with solid state fermentation SSF . Solid waste from rice bran used as the basic nutrient source. Supplementation with carbon and metal salt sources decrased the enzyme production. Certain fermentation parameters involving incubation time, incubation temperature, inoculum level, initial pH and extraction medium were studied separately. Maximal amount of α-amylase production 2716.9 ± 35.9 U/mg was obtained inoculum level 25%, initial pH 7.5 at 37oC for 48 h with supplementation of ammonium chloride.

Keywords:

Bacillus circulans ATCC 4516, rice bran, α-amylase, solid state fermentation,

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