Jelatin/Okside Dekstran Kriyojeller: In-Vitro Biyouyumluluk Değerlendirilmesi

B ir dokunun geliştirilmesi uygun bir destek/matriks dokusuna uygun hücreler ve sinyal moleküllerinin implantasyonuna gereksinim duyar. Doku ex-vivo doku oluşumunda destek malzemeleri olan doku iskeleleri biyolojik olarak uygun yapılar oluşturmak için belirli özelliklere sahip olmaları gereklidir. Bu çalışmada 3B’lu polimer jelatin/okside dekstran doku iskeleleri kriyojelasyon tekniği ile hazırlanmıştır. Hücre-doku iskelesi etkileşimleri Taramalı Elektron Mikroskobu ve Konfokal Lazer Mikroskobisi ile incelenmiştir. Doku iskelelerinin biyo ve kan uyumlulukları ile olası sitotoksik etkileri ayrıca incelenmiştir. Olası genotoksik etkileri DNA parçalanması be kırılması ile analiz edilmiştir. Sonuçlarımız göstermektedir ki, kriyojelasyon ile hazırlanan bu jelatin/ok-dekstran doku iskeleleri sitotoksik ve genotoksik etki göstermemişlerdir ve yüksek biyouyumluluk ve kan uyumluluğu ve kondrosit hücre etkileşimi göstermişlerdir

Anahtar Kelimeler:

Jelatin/okside-dekstran kriyojeller, Primer kondrositler, Biyouyumluluk

Gelatin/Oxide-Dextran Cryogels: In-Vitro Biocompatibility Evaluations

Engineering a tissue requires implantation of a suitable support/matrix material; seeded with appropriate cells and signaling molecules. Scaffolds which are the support material for the ex-vivo tissue formation, should have desired properties to form a biologically compatible constructs. In this recent study 3D polymeric gelatin/oxide dextran scaffolds were prepared by cryogelation. The cell-scaffold interactions were evaluated by Scanning Electron Microcopy and Confocal Laser Microscopy. Biocompatibility and haemocompatibility of the scaffolds and possible cytotoxicity were also investigated. Possible genotoxic effects of the scaffolds were evaluated with DNA fragmentation and breakage. Our results demonstrated that, gelatin/ox-dextran scaffolds which were prepared by cryogelation present no cytotoxicity and genotoxicity, show higher bio and haemocompatibility with excellent chondrocyte interaction.

Keywords:

Gelatin/oxide-dextran cryogels, Primary chondrocytes, biocompatibility,

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