Hüseyin Alkan

Poli(hidroksietil metakrilat-N-metakrilo-L-Histidin metil ester) Temelli Kriyogel ile Beta talasemili İnsan Plazmasından$Fe^{3+}$’ün Uzaklaştırması

Bu çalışmada, $Fe^{3+}$ adsorpsiyonu, süpermakrogözenekli poli(2-hidroksietil metakrilat-N-metakrilo-(I)-histidin metil ester) [poli(HEMA-MAH)] kriyojeli kullanılarak incelenmiştir. Poli(HEMA-MAH) kriyojeli elektron mikroskop taraması ve şişme testleriyle karakterize edilmiştir. Akış hızı, pH, $Fe^{3+}$’ün başlangıç derişiminin $Fe^3$ adsorpsiyonuna etkileri incelenmiştir. Poli(HEMA-MAH) temelli kriyojelin denge şişme derecesi 7.54 g $H_2$0/g kriyojel olarak bulunmuştur. Poli(HEMA-MAH) temelli kriyojelin $Fe^{3+}$ adsorpsiyon kapasitesi 1.71 mg/g bulunmuştur. Önemli miktarda adsorpsiyon kapasitesi kaybı olmadan $Fe^{3+}$ iyonlarının P(HEMA-MAH) kriyojel ile adsorbe ve desorbe edildiği saptanmıştır.

Poly(hydroxyethyl methacrylate)-co-N-methacryloyl-(L)- histidine methyl ester Based Cryogel for the Removal of $Fe^{3+}$ from Human Plasma effected with Beta Thalassemia

In this study, $Fe^{3+}$ adsorption was carried by using supermacroporous poly(2-hydroxyethyl methacrylate-Nmethacryloyl-(L)-histidine methyl ester) [poly(HEMA-MAH)] cryogel. Poly(HEMA-MAH) cryogel was characterized by swelling tests and scanning electron microscopy. The influence of flow rate, pH, initial $Fe^{3+}$ concentration, and $Fe^{3+}$ adsorption from human plasma effected with beta thalassemia on the adsorption efficiency of the cryogel was investigated. The equilibrium swelling degree of poly(HEMA-MAH) cryogel was found to be 7.54 g $H_2$O/g cryogel.$Fe^{3+}$ adsorption capacity of poly(HEMA-MAH) cryogel from aqueous solution was estimated as 1.79 mg/g cryogel, while lower adsorption capacity was observed in human plasma (1.71 mg/g). It was also observed that $Fe^{3+}$ could be repeatedly adsorbed and desorbed using poly(HEMA-MAH) cryogel without significant loss in its adsorption capacity.

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1. D. Purves, Trace Element Contamination of the Environment, Elsevier Science Publishers BV, Amsterdam, 1985.
2. B. Tang, L. Zhang, J. Zhang, Z. Z. Chen, Y.Wang, Spectrochim. Acta, 60 (2004) 2425.
3. M.C. Rouan, F. Marfil, P. Mangoni, R. Sechaud, H.Humbert, G. Maurer,. J. Chromatogr., B, 755 (2001) 203.
4. S. Ozkara, R. Say, C. Andac, Ind. Eng. Chem. Res., 47 (2008) 7849-7856
5. A. Denizli, B. Salih, E. Piskin, J. Biomater. Sci. Polym. Ed., 9 (1998) 175-187.
6. H. Yavuz, Y. Arica, A. Denizli, J. Appl.Polym. Sci., 82 (2001) 186-194.
7. M. Andac, E. Ozyapi, S. Senel, R. Say, A. Denizli, Ind. Eng.Chem. Res., 45 (2006) 1780-1786.
8. G. Baydemir, M. Andac, N. Bereli, R. Say, A. Denizli, Ind. Eng. Chem. Res., 46 (2007) 2843-2852.
9. M. McCoy, K. Kalghatgi, F.E. Regnier, N. Afeyan, J. Chromatogr. A, 743 (1996) 221–229.
10. E.B. Altıntas, A. Denizli, J. Chromatogr. B, 832 (2006) 216–223.
11. L. Uzun, R. Say, A. Denizli, React. Funct. Polym., 64 (2005) 93–102.
12. K. Thommes, M.R. Kula, Biotechnol. Prog., 11 (1995) 357–367.
13. T.B. Tennikova, B.J. Reusch, J. Chromatogr. A, 1065 (2005) 13–17.
14. N. Bereli, M. Andac , G. Baydemir, R. Say, I.Y. Galaev, A. Denizli, J. Chromatogr. A, 1190 (2008) 18–26.
15. F. Yılmaz, N. Bereli, H. Yavuz, A. Denizli, Biochem. Eng. J., 43 (2009) 272–279.
16. H. Alkan, N. Bereli, Z. Baysal, A. Denizli, Biochem. Eng. J., 45 (2009) 201–208.
17. G. Baydemir, N. Bereli, M. Andac , R. Say, I.Y. Galaev, A. Denizli, React. Funct. Polym., 69 (2009) 36–42.
18. G. Baydemir, N. Bereli, M. Andac , R. Say, I.Y. Galaev, A. Denizli, Colloid Surf. B, 68 (2009) 33–38.
19. L. Uzun, H. Yavuz, R. Say, A. Ersoz, A. Denizli, Ind. Eng. Chem. Res., 43 (2004) 6507–6513.
20. A. Cavus, Z. Baysal, H. Alkan, Colloids and Surfaces B: Biointerfaces, 107 ( 2013) 84–89
21. V.I. Lozinsky, I.Y. Galaev, F.M. Plieva, I.N. Savina, H. Jungvid, B. Mattiasson, Trends Biotechnol., 21 (2003) 445–451.
22. P. Arvidsson, F.M. Plieva, V.I. Lozinsky, I.Y. Galaev, B. Mattiasson, J. Chromatogr., A 986 (2003) 275–290.
23. V.I. Lozinsky, F.M. Plieva, I.Y. Galaev, B. Mattiasson, Bioseparation 10 (2002) 163–188.
24. W. Noppe, F.M. Plieva, K. Vanhoorelbeke, H. Deckmey, M. Tuncel, A.Tuncel, I.Y. Galaev, B. Mattiasson, J. Biotechnol., 131 (2007) 293–299.
25. P. Arvidsson, F.M. Plieva, I.N. Savina, V.I. Lozinsky, S. Fexby, L. Bulow, I.Y. Galaev, B. Mattiasson, J. Chromatogr. A, 977 (2002) 27–38.
26. B. Garipcan, A. Denizli, Macromol. Biosci., 2 (2002) 135-144.
27. E. Valdman, L. Erijman, F.L.P. Pessoa, S.G.F. Leite, Process Biochem., 36 (2001) 869-873.
28. M. Odabaşı, G. Baydemir, M. Karataş, A. Derazshamshir, J. Appl. Polym. Sci., 116 (2010) 1306-1312.
29. P. Girot, E. Averty, I. Flayeuz, E. Boschetti, J. Chromatogr. B., 808 (2004) 25-33.
30. Z.P. Liang, Y.Q. Feng, Z.Y. Liang, S.X. Mang, Biochemical Eng. Journal., 24 (2005) 65-72.
31. H. Sun, B. Ge, S. Liu, H. Chen, J. Sep. Sci., 31 (2008) 1201.