Electrochemical Characterization of Carbonized Typha Tassel Modified Screen-Printed Electrode and Its Enzymatic Glucose Oxidation Application

Electrochemical Characterization of Carbonized Typha Tassel Modified Screen-Printed Electrode and Its Enzymatic Glucose Oxidation Application

Converting biomass into cheaper but valuable products is very important for a more sustainable world. Especially with emerging technology, the use of hazardous materials in the synthesis of substances such as carbonaceous materials pose a threat to our environment. In this study, electrochemical performance of a carbonaceous material synthesized from typha tassel using a simple and cheap method without any hazardous substances was investigated. It was then used as an enzyme immobilization material for electrochemical glucose oxidation to demonstrate its potential application in bioelectronics. Physi-cal and chemical characterization of raw typha tassel (RTT) and carbonized typha tassel (CTT) were performed using SEM and FTIR techniques. CTT, was then grounded into fine powder, dispersed in DMF and coated onto screen-printed electrodes (SPEs). CTT modified SPEs were electrochemically tested using cyclic voltammetry in 0.1 M phosphate buffer containing 1 mM ferrocene carboxylic acid as a redox mediator at pH 7.4 Finally, glucose oxidase enzyme was adsorbed on CTT modified SPEs to demonstrate its performance in electrochemical enzymatic glucose oxidation reactions. SPE/CTT/GOx system showed promising electrochemical activity and stability at physiological conditions as well as good activity with adsorbed enzyme. This study suggests that CTT is very promising for an easy, effective and cheap ‘biomass to bioelectronics’ construction material.

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  • 1. H. Dai, Carbon Nanotubes: Synthesis, Integration, and Properties, Acc. Chem. Res., 35 (2002) 1035-1044.
  • 2. I. Kondratowicz, M. Nadolska, S. Şahin, M. Łapiński, M. Prześniak-Welenc, M. Sawczak, H.Y. Eileen, W. Sadowski, and K. Żelechowska, Tailoring properties of reduced graphene oxide by oxygen plasma treatment, Appl. Surf. Sci., 440 (2018) 651-659.
  • 3. S. Yang, Y. Li, S. Wang, M. Wang, M. Chu, and B. Xia, Advances in the use of carbonaceous materials for the electrochemical determination of persistent organic pollutants. A review, Microchim. Acta, 185 (2018) 112.
  • 4. Y. Gao, L. Li, Y. Jin, Y. Wang, C. Yuan, Y. Wei, G. Chen, J. Ge, and H. Lu, Porous carbon made from rice husk as electrode material for electrochemical double layer capacitor, Appl. Energy, 153 (2015) 41-47.
  • 5. A.M. Stephan, T.P. Kumar, R. Ramesh, S. Thomas, S.K. Jeong, and K.S. Nahm, Pyrolitic carbon from biomass precursors as anode materials for lithium batteries, Mater. Sci. Eng. A, 430 (2006) 132-137.
  • 6. M. Saxena and S. Sarkar, Synthesis of carbogenic nanosphere from peanut skin, Diam. Relat. Mater., 24 (2012) 11-14.
  • 7. N. Murugan and A.K. Sundramoorthy, Green synthesis of fluorescent carbon dots from Borassus flabellifer flowers for label-free highly selective and sensitive detection of Fe3+ions, New J. Chem., 42 (2018) 13297-13307.
  • 8. D.K. Kamysbayev, B. Serikbayev, G. Arbuz, G. Badavamova, and K.S. Tasibekov, Synthesis and Electrochemical Behavior of the Molybdenum-Modified Electrode Based on Rice Husk, Eurasian Chem.-Technol. J., 19 (2017) 315-321.
  • 9. D. Grieshaber, R. MacKenzie, J. Vörös, and E. Reimhult, Electrochemical Biosensors-Sensor Principles and Architectures, Sensors, 8 (2008) 1400.
  • 10. G.Z. Garyfallou, O. Ketebu, S. Şahin, E. Mukaetova-Ladinska, M. Catt, and E. Yu, Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer’s Disease, Sensors, 17 (2017) 2464.
  • 11. S. Sahin, T. Wongnateb, P. Chaiyenb, and E.H. Yu, Glucose Oxidation Using Oxygen Resistant Pyranose-2-Oxidase for Biofuel Cell Applications, Chem. Eng. Trans., 41 (2014)
  • 12. O.D. Renedo, M. Alonso-Lomillo, and M.A. Martínez, Recent developments in the field of screen-printed electrodes and their related applications, Talanta, 73 (2007) 202-219.
  • 13. A. Heller, Miniature biofuel cells, Phys. Chem. Chem. Phys., 6 (2004) 209-216.
  • 14. I. Willner, Y.M. Yan, B. Willner, and R. Tel-Vered, Integrated Enzyme -Based Biofuel Cells–A Review, Fuel Cells, 9 (2009) 7-24.
  • 15. R. Wilson and A.P.F. Turner, Glucose oxidase: an ideal enzyme, Biosens Bioelectron., 7 (1992) 165-185.
  • 16. G. Güven, S. Şahin, A. Güven, and E.H. Yu, Power Harvesting from Human Serum in Buckypaper-Based Enzymatic Biofuel Cell, Front. Energy Res., 4 (2016) 4.
  • 17. S. Şahin, J. Merotra, J. Kang, M. Trenell, M. Catt, and E.H. Yu, Simultaneous electrochemical detection of glucose and non-esterified fatty Acids (NEFAs) for diabetes management, IEEE Sens J., 18 (2018) 9075-9080
  • 18. S. Şahin, T. Wongnate, L. Chuaboon, P. Chaiyen, and E.H. Yu, Enzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalyst, Biosens Bioelectron., 107 (2018) 17-25.
  • 19. E.H. Yu and K. Scott, Enzymatic biofuel cells-fabrication of enzyme electrodes, Energies, 3 (2010) 23.
  • 20. S. Ferri, K. Kojima, and K. Sode, Review of glucose oxidases and glucose dehydrogenases: a bird’s eye view of glucose sensing enzymes, J. Diabetes Sci. Technol., 5 (2011) 1068-1076
  • 21. I. Ivanov, T. Vidaković-Koch, and K. Sundmacher, Recent Advances in Enzymatic Fuel Cells: Experiments and Modeling, Energies, 3 (2010) 803.
  • 22. J.K. Harkness, O.J. Murphy, and G.D. Hitchens, Enzyme electrodes based on ionomer films coated on electrodes, Journal of Electroanalytical Chemistry, 357 (1993) 261-272.
  • 23. P. Stepnicka, Ferrocenes: ligands, materials and biomolecules, John Wiley & Sons, USA, 2008.
  • 24. A. Erkal, İ. Aşık, S. Yavuz, A. Kariper, and Z. Üstündağ, Biosensor application of carbonaceous nanocoil material: preparation, characterization, and determination of dopamine and uric acid in the presence of ascorbic acid, J. Electrochem. Soc., 163 (2016) H269-H277.
  • 25. B. Abderrahim, E. Abderrahman, M. Aqil, T. Fatima, T. Abdesselam, and O. Krim, Kinetic Thermal Degradation of Cellulose, Polybutylene Succinate and a Green Composite: Comparative Study, 2015
  • 26. M. Naebe, J. Wang, A. Amini, H. Khayyam, N. Hameed, L.H. Li, Y. Chen, and B. Fox, Mechanical property and structure of covalent functionalised graphene/epoxy nanocomposites, Sci. Rep., 4 (2014) 4375.
  • 27. X.H. Pham, M.P.N. Bui, C.A. Li, K.N. Han, J.H. Kim, H. Won, and G.H. Seong, Electrochemical characterization of a single-walled carbon nanotube electrode for detection of glucose, Analyt. chimica acta, 671 (2010) 36-40.