Functional Polysaccharides Blended Collagen Cryogels

Researches investigate new types of scaffolds for tissue engineering and regenerative medicine applications to support cell proliferation and growth as well as tissue repair and regeneration. Although, there are several types of polymeric materials and various kinds of preparation techniques are already used today; there is still no consensus on the answer of the question “what is the best scaffold for tissue repair and regeneration?”. Cryogels, which is a kind of hydrogels and, cryogelation, which is the technique for cryogel preparation, are rather new for tissue engineering applications. Here in this study, dextran and carboxymethyl cellulose functional polysaccharides blended collagen cryogels were prepared and characterized by chemical, structural and biological evaluations. Results show that, these collagen cryogels with their polysaccharides functional components have proper chemical and thermal characteristics and show good bio and hemocompatibility. Therefore, these cryogels can be used as a candidate scaffold for tissue engineering and regenerative medicine applications.

Keywords:

Collagen, polysaccharides, cryogels tissue engineering,

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R.M. Nerem, A. Sambanis, Tissue engineering: from biology to biological substitutes, Tissue Eng., 1 (1995) 3-13.
S. Yang, K.F. Leong, Z. Du, C.K. Chua, The design of scaffolds for use in tissue engineering. Part I. Traditional factors, Tissue Eng., 7 (2001) 679-689.
L.E. Freed, G. Vunjak-Novakovic, R.J. Biron, D.B. Eagles, D.C. Lesnoy, S.K. Barlow, R. Langer, Biodegradable polymer scaffolds for tissue engineering, Biotechnol., 12 (1994) 689-693.
B.P. Chan, K.W. Leong, Scaffolding in tissue engineering: general approaches and tissue-specific considerations, European spine journal: official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society 17 Suppl. 4 (2008) 467-479.
S.J. Hollister, R.D. Maddox, J.M. Taboas, Optimal design and fabrication of scaffolds to mimic tissue properties and satisfy biological constraints, Biomaterials, 23 (2002) 4095-4103.
V.I. Lozinsky, I.Y. Galaev, F.M. Plieva, I.N. Savina, H. Jungvid, B. Mattiasson, Polymeric cryogels as promising materials of biotechnological interest, Trends Biotechnol., 21 (2003) 445-451.
M. Andac, I.Y. Galaev, H. Yavuz, A. Denizli, Molecularly imprinted cryogels for human serum albumin depletion in: Affinity Chromatography: Methods and Protocols, S. Reichtelt, ed., Methods in Molecular Biology Series, volume 1286, pp 233-237, Humana Press, 2015.
D. Cimen, F. Yilmaz, I. Percin, D. Turkmen, A. Denizli, Dye affinity cryogels for plasmid DNA purification, Mater. Sci. Eng. Mater. Biol. Appl., 56 (2015) 318-324.
A. Kumar, A. Bhardwaj, Methods in cell separation for biomedical application: cryogels as a new tool, Biomed. Mater., 3 (2008) 1-12.
M. Uygun, A.A. Karagozler, A. Denizli, Molecularly imprinted cryogels for carbonic anhydrase purification from bovine erythrocyte, Artif. Cells Nanomed. Biotechnol., 42 (2014) 128-137.
N. Bolgen, I. Vargel, P. Korkusuz, E. Guzel, F. Plieva, I. Galaev, B. Matiasson, E. Piskin, Tissue responses to novel tissue engineering biodegradable cryogel scaffolds: an animal model, J. Biomed. Mater. Res. A, 91 (2009) 60-68.
C.H. Chen, C.Y. Kuo, Y.J. Wang, J.P. Chen, Dual function of glucosamine in gelatin/hyaluronic acid cryogel to modulate scaffold mechanical properties and to maintain chondrogenic phenotype for cartilage tissue engineering, Int. J. Mol. Sci., 17 (2016) 1-22.
M.V. Konovalova, P.A. Markov, E.A. Durnev, D.V. Kurek, S.V. Popov, V.P. Varlamov, Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application, J. Biomed. Mater. Res. A, 105 (2017) 547-556.
S. Odabas, G.A. Feichtinger, P. Korkusuz, I. Inci, E. Bilgic, A.S. Yar, T. Cavusoglu, S. Menevse, I. Vargel, E. Piskin, Auricular cartilage repair using cryogel scaffolds loaded with BMP-7-expressing primary chondrocytes, J. Tissue Eng. Regen. Med., 7 (2013) 831-840.
A. Sharma, S. Bhat, T. Vishnoi, V. Nayak, A. Kumar, Three-dimensional supermacroporous carrageenangelatin cryogel matrix for tissue engineering applications, Bio. Med. Res. Int., X (2013) 1-15.
L. Cen, W. Liu, L. Cui, W. Zhang, Y. Cao, Collagen tissue engineering: development of novel biomaterials and applications, Pediatric Res., 63 (2008) 492-496.
P. Fratzl, K. Misof, I. Zizak, G. Rapp, H. Amenitsch, S. Bernstorff, Fibrillar structure and mechanical properties of collagen, J. Struc. Biol., 122 (1998) 119- 122.
L. Ma, C. Gao, Z. Mao, J. Zhou, J. Shen, X. Hu, C. Han, Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering, Biomaterials, 24 (2003) 4833-4841
M. Rafat, F. Li, P. Fagerholm, N.S. Lagali, M.A. Watsky, R. Munger, T. Matsuura, M. Griffith, PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering, Biomaterials, 29 (2008) 3960-3972.
S. Mavila, O. Eivgi, I. Berkovich, N.G. Lemcoff, Intramolecular cross-linking methodologies for the synthesis of polymer nanoparticles, Chem. Rev., 116 (2016) 878-961.
R. Kluger, A. Alagic, Chemical cross-linking and protein-protein interactions-a review with illustrative protocols, Bioorganic Chem., 32 (2004) 451-472.
R.A.C. João Maia, Jorge F.J. Coelho, Pedro N.Simões, M. Helena Gil, Insight on the periodate oxidation of dextran and its structural vicissitudes, Polymer, 52 (2011) 258-265.
Y.D. Czaja WK, Kawecki M, Brown RM Jr., The future prospects of microbial cellulose in biomedical applications, Biomacromol., 8 (2007) 1-12.
N.A. Hoenich, Cellulose for medical applications: past, present, and future, BioResources, 1 (2006) 1-11.
B. León-Mancilla, M. Araiza-Téllez, J. Flores-Flores, M. Piña-Barba, Physico-chemical characterization of collagen scaffolds for tissue engineering, J. Appl. Res. Tech., 14 (2016) 77-85.
P.R.F.D.S. Moraes, S. Saska, H. Barud, L.R.D. Lima, V.D.C.A. Martins, A.M.D.G. Plepis, S.J.L. Ribeiro, A.M.M. Gaspar, Bacterial cellulose/collagen hydrogel for wound healing, Mater. Res., 19 (2016) 106-116.
T. Nagai, N. Suzuki, Y. Tanoue, N. Kai, Collagen from tendon of Yezo Sika deer (Cervus nippon yesoensis) as By-Product, Food Nutr. Sci., 3 (2012) 72-79.
J. Autian, Biological model systems for the testing of the toxicity of biomaterials, Polymers in Medicine and Surgery, Springer, Boston, MA, 1975.

Hacettepe Journal of Biology and Chemistry-Cover

ISSN: 2687-475X
Yayın Aralığı: Yılda 4 Sayı
Başlangıç: 1972
Yayıncı: Hacettepe Üniversitesi, Fen Fakültesi

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