Hatem KAZIMOĞLU, Halil Mahir KAPLAN

Haplophyllum Buxbaumii Ekstresinin Prostat Kanseri Üzerindeki Apoptotik Etkisi

Prostat kanseri, erkekler arasında yaygın bir kanser türüdür. Son zamanlarda, kanser tedavisinde bitkisel kökenli yeni antikanser ajanlar üzerine yapılan çalışmalar artmıştır. Haplophyllum buxbaumii (HP) çok yıllık otsu bir bitkidir. Çalışmalar, Haplophyllum alt türlerinin antioksidan özelliklere sahip olduğunu bildirmiştir. HP'nin antikanser etkisi bilinmemektedir. Bu çalışma, HP'nin prostat kanseri üzerindeki apoptotik etkisini ortaya çıkarmayı amaçlamaktadır. HP'nin sitotoksik etkisi MTT yöntemi ile değerlendirildi. Ayrıca apoptotik yol aracı medyatörleri ELISA yöntemi ile analiz edildi. HP ekstresi uygulaması, prostat kanserinin canlılığını azalttı ve apoptotik medyatörleri aktive ederken, anti-apoptotik mediyatörleri azalttı. Çalışmamız, HP ekstresinin prostat kanseri üzerinde antiproliferatif ve apoptotik bir etkiye sahip olduğunu gösterdi.

Anahtar Kelimeler:

Haplophyllum buxbaumii, Apopitozis, Sitotoksisite, Prostat kanseri

Apoptotic Effect of Haplophyllum Buxbaumii Extract on Prostate Cancer

Prostate cancer is a common type of cancer among men. Recently, studies on new anticancer agents of herbal origin in the treatment of cancer have increased. Haplophyllum buxbaumii (HP) is a perennial herbaceous plant. Studies have reported that Haplophyllum subspecies has antioxidant properties. And the anticancer effect of HP is unknown. This study aims to reveal the apoptotic effect HP on prostate cancer. The cytotoxic effect of HP was evaluated by the MTT method. In addition, apoptotic pathway mediators were analyzed by ELISA method. HP extract application reduced the viability of prostate cancer and activated the apoptotic mediators while decreasing anti-apoptotic mediators. Our study showed that HP extract has an antiproliferative and apoptotic effect on prostate cancer.

Keywords:

Haplophyllium buxbaumii, Apoptosis, Cytotoxicity, Prostate cancer,

PDF

___

1. No authors listed. (2013). "Locally Advanced Prostate Cancer: Effective Treatments, But Many Adverse Effects". Prescrire Int., 22 (134), 18-20, 22-3 (ISSN: 1167-7422). https://pubmed. ncbi.nlm.nih.gov/23367679/
2. Miranda-Goncalves, V., Lameirinhas, A., Henrique, R. and Jeronimo, C. (2019) "Metabolism and Epigenetic Interplay in Cancer: Regulation and Putative Therapeutic Targets". Front Genet, 9 (10), 427.
3. Prieto, J.M., Recio, M.C., Giner, R.M, Manez, S., Massmanian, A., Waterman, P.G. and Rios, J.L. (1996). "Topical Anti-Inflammatory Lignans From Haplophyllum Hispanicum". Biosci, 51 (9-10), 618-622.
4. Kaplan, H.M., Singirik, E., Erdogan, K.E. and Doran, F. (2017). "Protective Effect of Alpha-Linolenic Acid on Gentamicin-Induced Ototoxicity in Mice". Somatosens Mot Res., 34 (3), 145-150.
5. Kaplan, H.M., Izol, V., Aridogan, I.A., Olgan, E., Yegani, A.A., Pazarci, P. and Singirik, E. (2016). "Protective Effect of Hypericum Perforatum Extract on Gentamicin Induced Nephrotoxicity in Mice." Int J Pharmacol, 12 (6), 663-668.
6. Izol, V., Aridogan, I.A., Tansug, Z., Doran, F., Erdogan, K.E., Kaplan, H.M., Singirik, E., Ertug, P. and Pazarci, P. (2019) "Hypericum Perforatum Extract Attennuates Gentamicin Induced Oxidative Stress, Apoptosis and Oedema in Kidney". Int J Pharmacol, 15 (1), 66-73.
7. Choromanska, A., Kulbacka, J., Harasym, J., Dubinska-Magiera, M. and Saczko, J. (2017). "Anticancer Activity of Oat Β-Glucan in Combination with Electroporation on Human Cancer Cells". Acta Pol. Pharm. Drug Res, 74, 616-623.
8. Kocyigit, A., Koyuncu, I., Dikilitas, M., Bahadori, F. and Turkkan, B. (2016). "Cytotoxic, Genotoxic and Apoptotic Effects of Naringenin-Oxime Relative to Naringenin on Normal and Cancer Cell Lines". Asian Pac J Trop Bio, 6 (10), 872-880.
9. Buyel, J.F. (2018). "Plants As Sources of Natural and Recombinant Anti-Cancer Agents". Biotechnology Advances, 36 (2), 506-520.
10. Jain, K.K. (2007). "Cancer Biomarkers: Current Issues and Future Directions". Curr Opin Mol Ther, 9 (6), 563-571.
11. Sun, .J., Wei, Q., Zhou, Y., Wang, J., Liu, Q. and Xu, H. (2017). "A Systematic Analysis of FDA-Approved Anticancer Drugs". BMC Syst Biol, 11 (Suppl 5), 87.