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Glikoz İzomeraz Üreticisi Dört Geobacillus Suşunun İzolasyonu ve Moleküler Metotlar Kullanarak Tanımlanması

Türkiye'nin batısındaki iki kaplıca, termofilik mikroorganizmaların mevcudiyetine göre araştırıldı ve iki termal kaplıcadan,23 termofilik bakteri izole edildi. İzolatlardan, 4 tanesinin glikoz izomeraz üretimi açısından pozitif olduğu belirlendi. BT5, CT6SARI ve CT1.2 izolatlarının glikoz izomerazları, pH 6.5'te optimum büyüme gösterdi; buna karşılık izolat BT1 enzimi, pH 7.5'te optimal aktivite gösterdi. Bu enzimler arasında BT1 izolatının optimal büyüme sıcaklığı 75 °C olarak belirlenirken, diğer üç izolatın enzimleri ise 80 °C'de optimal büyüme gösterdi. 16S rRNA gen dizi analizleri ile 4 izolatın, Geobacillus cinsine ait türler oldukları belirlendi. recN dizisi benzerlik analizine dayanarak, izolat CT6SARI’nın, G. termodenitficans’a; izolat BT1’in de, G. stearothermophilus’a ait suşlar oldukları belirlendi.BT5 ve CT1.2 izolatlarının kesin tür tayinleri yapılamadı.

Anahtar Kelimeler:

Geobacillus, Glikoz izomeraz, recN, 16S rRNA

Isolation of Four Glucose Isomerase Producing Strains of Geobacillus and Their Identification Using Molecular Methods

Two hot springs located in west of Turkey were investigated with respect to presence of thermophilic microorganisms. 23 thermophilic bacteria were isolated from two hot springs. Among these, 4 strains were found positive for glucose isomerase production. The glucose isomerases of strains BT5, CT6SARI and CT1.2 showed optimal growth at pH 6.5, whereas the enzyme of strain BT1 represented optimal activity at pH 7.5. The strain BT1 had the lowest temperature optima (75°C) among these strains whereas the other three strains showed optimal growth at 80 °C. On the basis of the 16S rRNA gene sequence analysis, the 4 isolates are members of the genus Geobacillus. Based on recN sequence similarity analysis, the isolate CT6SARI is a strain of G. thermodenitficans. The isolate BT1 is a strain of G. stearothermophilus. The species identity for the isolates BT5 and CT1.2are uncertain.

Keywords:

Geobacillus, Glucose-isomerase, recN, 16S rRNA,

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