YANIT YÜZEY TASARIMI KULLANILARAK KİVİ MEYVESİNDEKİ MAKSİMUM FENOLİK BİLEŞİKLER İÇİN EKSTRAKSİYON KOŞULLARININ BELİRLENMESİ

Bu çalışmanın ana odak noktası yanıt yüzey metodolojisi (RSM) kullanarak kivi meyvesinden olabildiğince fazla fenolik bileşik ve antioksidan aktivite için ekstraksiyon parametrelerini belirlemektir. Ekstraksiyon parametrelerinin etkisini belirlemek için RSM’de Box-Behnken deneysel tasarımı kullanıldı. Metanol konsantrasyonu (A: %60-75-90), ultrason süresi (B: 5-10-15 dakika) ve numune kütlesi (C: 1-2-3 g) olmak üzere üç seviyede üç bağımsız değişken seçildi. Bu şartlar altında maksimum fitokimyasal verim için koşullar A = % 90 metanol konsantrasyonu, B = 7.71 dakika ve C = 3 g olarak bulundu. Bu koşullar altındaki ekstraksiyon sonucu kivide DPPH % 88.96, ABTS 739.07 mg/100 g ve toplam fenolik içerik 299.14 mg/100 g tespit edildi.

Anahtar Kelimeler:

Kivi, Yanıt Yüzey Metodolojisi, Toplam Fenolik Madde, antioksidan aktivite

DETERMINATION EXTRACTION CONDITIONS FOR MAXIMUM PHENOLIC COMPOUNDS IN THE KIWIFRUIT USING RESPONSE SURFACE DESIGN

The study aims to determine the extraction parameters for maximal phenolic compounds and antioxidant activity from kiwifruit by using response surface methodology (RSM). Box-Behnken experimental design of RSM was applied to determine the effect of extraction parameters. Three independent variables were selected at three levels: methanol concentration (A: 60-75-90 %), ultrasound time (B: 5-10-15 min.), and mass of sample (C: 1-2-3 g). Conditions for maximum phenolic yield were found as A = 90%, B= 7.71 min, and C = 3 g, under this condition, DPPH of 88.96 %, ABTS of 739.07 mg/100g FW and TPC of 299.14 mg/100g fresh weight has been obtained.

Keywords:

Kiwifruit, Response Surface Methodology, Total Phenolic Matter, antioxidant activity,

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